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Literature DB >> 6263759

SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen.

Abstract

A modification of the Graham and Van der Eb (1974) DNA-calcium phosphate coprecipitation technique is shown to routinely transfect 15% of CV-1 cells with SV40 DNA. The transfection is done in suspension after detachment of cells by trypsin digestion. Transfection efficiency was measured by staining cells for the presence of SV40 T-antigen by indirect immunofluorescence and by assaying for the presence of SV40 early message by the Berk and Sharp (1978) technique.

Entities: Chemical

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Year: 1981 PMID： 6263759 DOI： 10.1016/0378-1119(81)90008-1

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

51 in total

1. A new type of insertion mutation in monkey cells: insertion accompanied by long target site duplication.

Authors: M Ohira; Y S Bae; H Ikeda
Journal: Mol Gen Genet Date: 1991-10

Review 2. Large-scale transfection of mammalian cells for the fast production of recombinant protein.

Authors: Phuong Lan Pham; Amine Kamen; Yves Durocher
Journal: Mol Biotechnol Date: 2006-10 Impact factor: 2.695

3. p48 Activates a UV-damaged-DNA binding factor and is defective in xeroderma pigmentosum group E cells that lack binding activity.

Authors: B J Hwang; S Toering; U Francke; G Chu
Journal: Mol Cell Biol Date: 1998-07 Impact factor: 4.272

4. Expression of transfected DNA in avian cells can be enhanced in trans by retroviral infection.

Authors: B R Cullen; R A Katz; G Ju
Journal: Mol Cell Biol Date: 1985-07 Impact factor: 4.272

5. Different relative expression from two murine leukemia virus long terminal repeats in unintegrated transfected DNA and in integrated retroviral vector proviruses.

Authors: K Paludan; H Y Dai; M Duch; P Jørgensen; N O Kjeldgaard; F S Pedersen
Journal: J Virol Date: 1989-12 Impact factor: 5.103

10. Homologous recombination of monkey alpha-satellite repeats in an in vitro simian virus 40 replication system: possible association of recombination with DNA replication.

Authors: I Kawasaki; Y S Bae; T Eki; Y Kim; H Ikeda
Journal: Mol Cell Biol Date: 1994-06 Impact factor: 4.272

SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen.

1. A new type of insertion mutation in monkey cells: insertion accompanied by long target site duplication.

Review 2. Large-scale transfection of mammalian cells for the fast production of recombinant protein.

3. p48 Activates a UV-damaged-DNA binding factor and is defective in xeroderma pigmentosum group E cells that lack binding activity.

4. Expression of transfected DNA in avian cells can be enhanced in trans by retroviral infection.

5. Different relative expression from two murine leukemia virus long terminal repeats in unintegrated transfected DNA and in integrated retroviral vector proviruses.

6. GT to AT transition at a splice donor site causes skipping of the preceding exon in phenylketonuria.

7. Expression of an immunoglobulin heavy chain gene transfected into lymphocytes.

8. Expression and regulation of chicken actin genes introduced into mouse myogenic and nonmyogenic cells.

9. Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains.

10. Homologous recombination of monkey alpha-satellite repeats in an in vitro simian virus 40 replication system: possible association of recombination with DNA replication.