Literature DB >> 6092133

A gene fusion that localises the penicillin-binding domain of penicillin-binding protein 3 of Escherichia coli.

P J Hedge, B G Spratt.   

Abstract

A gene fusion that links the COOH-terminal 349 amino acids of penicillin-binding protein 3 (60 kDa) of E. coli to the NH2-terminus of beta-galactosidase has been constructed. The fusion protein (38.5 kDa) retains the ability to bind benzylpenicillin with high affinity, establishing that the penicillin-binding domain (and presumably the penicillin-sensitive transpeptidase activity) of this high molecular mass penicillin-binding protein is located on a COOH-terminal functional domain.

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Year:  1984        PMID： 6092133     DOI： 10.1016/0014-5793(84)80936-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  10 in total

1.  Determination of the cleavage site involved in C-terminal processing of penicillin-binding protein 3 of Escherichia coli.

Authors:  H Nagasawa; Y Sakagami; A Suzuki; H Suzuki; H Hara; Y Hirota
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

2.  Escherichia coli mutant Y16 is a double mutant carrying thermosensitive ftsH and ftsI mutations.

Authors:  K J Begg; T Tomoyasu; W D Donachie; M Khattar; H Niki; K Yamanaka; S Hiraga; T Ogura
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

3.  The non-penicillin-binding module of the tripartite penicillin-binding protein 3 of Escherichia coli is required for folding and/or stability of the penicillin-binding module and the membrane-anchoring module confers cell septation activity on the folded structure.

Authors:  C Goffin; C Fraipont; J Ayala; M Terrak; M Nguyen-Distèche; J M Ghuysen
Journal:  J Bacteriol       Date:  1996-09       Impact factor: 3.490

4.  Identification of the active site in penicillin-binding protein 3 of Escherichia coli.

Authors:  R A Nicholas; J L Strominger; H Suzuki; Y Hirota
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

5.  A lacZ-pbpB gene fusion coding for an inducible hybrid protein that recognizes localized sites in the inner membrane of Escherichia coli.

Authors:  J A Ayala; J Plá; L R Desviat; M A de Pedro
Journal:  J Bacteriol       Date:  1988-08       Impact factor: 3.490

6.  Binding of penicillin to thiol-penicillin-binding protein 3 of Escherichia coli: identification of its active site.

Authors:  N Houba-Hérin; H Hara; M Inouye; Y Hirota
Journal:  Mol Gen Genet       Date:  1985

7.  Sequences of the active-site peptides of three of the high-Mr penicillin-binding proteins of Escherichia coli K-12.

Authors:  W Keck; B Glauner; U Schwarz; J K Broome-Smith; B G Spratt
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

8.  Lack of relevance of kinetic parameters for exocellular DD-peptidases to cephalosporin MICs.

Authors:  D B Boyd; J L Ott
Journal:  Antimicrob Agents Chemother       Date:  1986-05       Impact factor: 5.191

9.  The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 DD-peptidase family.

Authors:  B Joris; J M Ghuysen; G Dive; A Renard; O Dideberg; P Charlier; J M Frère; J A Kelly; J C Boyington; P C Moews
Journal:  Biochem J       Date:  1988-03-01       Impact factor: 3.857

10.  Production of thiol-penicillin-binding protein 3 of Escherichia coli using a two primer method of site-directed mutagenesis.

Authors:  J K Broome-Smith; P J Hedge; B G Spratt
Journal:  EMBO J       Date:  1985-01       Impact factor: 11.598
  10 in total

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