Literature DB >> 6091041

Introns and their flanking sequences of Bombyx mori rDNA.

H Fujiwara, T Ogura, N Takada, N Miyajima, H Ishikawa, H Maekawa.   

Abstract

We obtained two different clones (16 kb and 13 kb) of B. mori rDNA with intron sequence within the 28S-rRNA coding region. The sequence surrounding the intron was found to be highly conserved as indicated in several eukaryotes (Tetrahymena, Drosophila and Xenopus). The 28S rRNA-coding sequence of 16 kb and 13 kb clone was interrupted at precisely the same sites as those where the D. melanogaster rDNA interrupted by the type I and type II intron, respectively. The intron sequences of B. mori were different from those of D. melanogaster. In 16 kb clone, the intron was flanked by 14 bp duplication of the junction sequence, which was also present once within the 28S rRNA-coding region of rDNA without intron. This 14 bp sequence was identical with those surrounding the introns of Dipteran rDNAs.

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Year:  1984        PMID： 6091041      PMCID： PMC320122          DOI： 10.1093/nar/12.17.6861

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  30 in total

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Authors:  R F Manning; D R Samols; L P Gage
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  22 in total

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