Literature DB >> 6090131

Spliced RNA from the IR1-U2 region of Epstein-Barr virus: presence of an open reading frame for a repetitive polypeptide.

M Bodescot, B Chambraud, P Farrell, M Perricaudet.   

Abstract

We have constructed a cDNA library from the cytoplasmic RNAs of Raji cells, a Burkitt's lymphoma cell line latently infected with Epstein-Barr virus. We report here the characterization of a cDNA representing a spliced RNA transcribed from the IR1-U2 region of the viral genome. The cDNA is 1007 bp long. The 5' region contains three tandem repeats of two exons, 66 and 132 bp, which are transcribed from the IR1 repeats. The 3' region is formed from four exons transcribed from U2. An open reading frame extends from the 5' end to position 784, and includes the repeats. This reading frame presumably corresponds to the carboxy-terminal 261 amino acids of a polypeptide containing several repeats of a 66 amino acid sequence. Since it would be encoded by the IR1-U2 region of the viral genome, the putative polypeptide might be involved in the process of growth-transformation of B-lymphocytes.

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Year:  1984        PMID： 6090131      PMCID： PMC557617          DOI： 10.1002/j.1460-2075.1984.tb02067.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  47 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Cleavage of Epstein-Barr virus DNA by restriction endonucleases EcoRI, HindIII and BamI.

Authors:  L Rymo; S Forsblom
Journal:  Nucleic Acids Res       Date:  1978-04       Impact factor: 16.971

4.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

5.  A procedure for the isolation of mammalian messenger ribonucleic acid.

Authors:  G Brawerman; J Mendecki; S Y Lee
Journal:  Biochemistry       Date:  1972-02-15       Impact factor: 3.162

6.  3' non-coding region sequences in eukaryotic messenger RNA.

Authors:  N J Proudfoot; G G Brownlee
Journal:  Nature       Date:  1976-09-16       Impact factor: 49.962

7.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

8.  Differences between laboratory strains of Epstein-Barr virus based on immortalization, abortive infection, and interference.

Authors:  G Miller; J Robinson; L Heston; M Lipman
Journal:  Proc Natl Acad Sci U S A       Date:  1974-10       Impact factor: 11.205

9.  Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line.

Authors:  Y Hinuma; M Konn; J Yamaguchi; D J Wudarski; J R Blakeslee; J T Grace
Journal:  J Virol       Date:  1967-10       Impact factor: 5.103

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  43 in total

1.  Epstein-Barr virus latent gene expression in uncultured peripheral blood lymphocytes.

Authors:  L Qu; D T Rowe
Journal:  J Virol       Date:  1992-06       Impact factor: 5.103

2.  Monoclonal and polyclonal antibodies against Epstein-Barr virus nuclear antigen 5 (EBNA-5) detect multiple protein species in Burkitt's lymphoma and lymphoblastoid cell lines.

Authors:  J Finke; M Rowe; B Kallin; I Ernberg; A Rosén; J Dillner; G Klein
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103

3.  Bidirectional transcription of the Epstein-Barr virus major internal repeat.

Authors:  R P Rogers; S H Speck
Journal:  J Virol       Date:  1990-05       Impact factor: 5.103

4.  The Epstein-Barr virus nuclear protein encoded by the leader of the EBNA RNAs is important in B-lymphocyte transformation.

Authors:  J B Mannick; J I Cohen; M Birkenbach; A Marchini; E Kieff
Journal:  J Virol       Date:  1991-12       Impact factor: 5.103

5.  Transcription of the Epstein-Barr virus genome during latency in growth-transformed lymphocytes.

Authors:  J Sample; E Kieff
Journal:  J Virol       Date:  1990-04       Impact factor: 5.103

6.  Regulation of the double-stranded RNA-dependent protein kinase PKR by RNAs encoded by a repeated sequence in the Epstein-Barr virus genome.

Authors:  A Elia; K G Laing; A Schofield; V J Tilleray; M J Clemens
Journal:  Nucleic Acids Res       Date:  1996-11-15       Impact factor: 16.971

7.  Interaction of the lymphocyte-derived Epstein-Barr virus nuclear antigen EBNA-1 with its DNA-binding sites.

Authors:  C H Jones; S D Hayward; D R Rawlins
Journal:  J Virol       Date:  1989-01       Impact factor: 5.103

8.  Sequences of the Epstein-Barr Virus (EBV) large internal repeat form the center of a 16-kilobase-pair palindrome of EBV (P3HR-1) heterogeneous DNA.

Authors:  H B Jenson; P J Farrell; G Miller
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

9.  Clustered alternative splice sites in Epstein-Barr virus RNAs.

Authors:  M Bodescot; M Perricaudet
Journal:  Nucleic Acids Res       Date:  1987-07-24       Impact factor: 16.971

10.  Epstein-Barr virus gene expression in P3HR1-superinfected Raji cells.

Authors:  M Biggin; M Bodescot; M Perricaudet; P Farrell
Journal:  J Virol       Date:  1987-10       Impact factor: 5.103
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