Human apolipoprotein A-II: nucleotide sequence of a cloned cDNA, and localization of its structural gene on human chromosome 1.

Using the technique of oligonucleotide hybridization, we have isolated two dscDNA clones to human apolipoprotein A-II. One of the clones (pAII-1) has been completely sequenced. It has 433 nucleotides which includes a poly A tail of 10 adenylic acid residues, all the coding and 3'-non-translated regions of the mRNA and part of the 5'-non-translated region. The amino acid sequence derived from the cDNA clone includes 100 amino acids (including the 23 amino acid prepropeptide) which is very similar to the sequence reported by Brewer et al. (Proc. Natl. Acad. Sci. USA 69, 1304-1308). [32P]-labeled pAII-1 was used as a probe in chromosome mapping studies to detect the human apoAII structural gene sequence in human-Chinese hamster cell hybrids. Southern blot analysis of 10 hybrids localized the gene to human chromosome 1.