| Literature DB >> 6086626 |
K Mizuuchi, M Mizuuchi, M H O'Dea, M Gellert.
Abstract
We have transferred the Escherichia coli gyrA and gyrB genes onto plasmids that allow the overproduction of the DNA gyrase A and B proteins and have designed relatively simple purification procedures for both proteins. The pure proteins are obtained in good yield; from 2 liters of culture (12 g of cells), one can recover 25 mg of GyrA or 3 mg of GyrB protein.Entities:
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Year: 1984 PMID： 6086626
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157