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Literature DB >> 6083561

Protein export in Escherichia coli requires a soluble activity.

Abstract

By using a reconstituted cell-free system we have demonstrated the existence of a soluble activity that is required for the export of proteins in Escherichia coli. This export factor sediments at about 12 S. It has been partially purified by passage through an omega-NH2-butylagarose column and by salt elution off a DEAE matrix. The export factor does not contain 6S RNA.

Entities: Chemical Species

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Year: 1984 PMID： 6083561 PMCID： PMC392227 DOI： 10.1073/pnas.81.24.7737

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

25 in total

1. Purification and characterization of protein synthesis initiation factors IF1, IF2, and IF3 from Escherichia coli.

Authors: J W Hershey; J Yanov; K Johnston; J L Fakunding
Journal: Arch Biochem Biophys Date: 1977-08 Impact factor: 4.013

2. Small stable RNAs from Escherichia coli: evidence for the existence of new molecules and for a new ribonucleoprotein particle containing 6S RNA.

Authors: S Y Lee; S C Bailey; D Apirion
Journal: J Bacteriol Date: 1978-02 Impact factor: 3.490

3. Studies on the in vitro synthesis of beta-galactosidase: necessary components in the ribosomal wash.

Authors: H F Kung; C Spears; T Schulz; H Weissbach
Journal: Arch Biochem Biophys Date: 1974-06 Impact factor: 4.013

4. Studies on the role of ribosomal proteins L 7 and L 12 in the in vitro synthesis of -galactosidase.

Authors: H F Kung; J E Fox; C Spears; N Brot; H Weissbach
Journal: J Biol Chem Date: 1973-07-25 Impact factor: 5.157

5. Sequence of 6S RNA of E. coli.

Authors: G G Brownlee
Journal: Nat New Biol Date: 1971-02-03

6. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

Authors: R R Burgess
Journal: J Biol Chem Date: 1969-11-25 Impact factor: 5.157

7. Isolation of the bacteriophage lambda receptor from Escherichia coli.

Authors: L Randall-Hazelbauer; M Schwartz
Journal: J Bacteriol Date: 1973-12 Impact factor: 3.490

8. Translocation of proteins across the endoplasmic reticulum. II. Signal recognition protein (SRP) mediates the selective binding to microsomal membranes of in-vitro-assembled polysomes synthesizing secretory protein.

Authors: P Walter; G Blobel
Journal: J Cell Biol Date: 1981-11 Impact factor: 10.539

9. Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.

Authors: P Walter; G Blobel
Journal: J Cell Biol Date: 1981-11 Impact factor: 10.539

10. Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein.

Authors: P Walter; I Ibrahimi; G Blobel
Journal: J Cell Biol Date: 1981-11 Impact factor: 10.539

39 in total

1. Escherichia coli SecB protein associates with exported protein precursors in vivo.

Authors: C A Kumamoto
Journal: Proc Natl Acad Sci U S A Date: 1989-07 Impact factor: 11.205

2. The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export.

Authors: P M Gannon; P Li; C A Kumamoto
Journal: J Bacteriol Date: 1989-02 Impact factor: 3.490

3. Temperature-dependent insertion of prolipoprotein into Escherichia coli membrane vesicles and requirements for ATP, soluble factors, and functional SecY protein for the overall translocation process.

Authors: G Tian; H C Wu; P H Ray; P C Tai
Journal: J Bacteriol Date: 1989-04 Impact factor: 3.490

4. Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors.

Authors: J P Fandl; P C Tai
Journal: Proc Natl Acad Sci U S A Date: 1987-11 Impact factor: 11.205

9. Nascent secretory polypeptides synthesized on Escherichia coli ribosomes are not translocated across mammalian endoplasmic reticulum.

Authors: I Ibrahimi; E Fuchs
Journal: J Bacteriol Date: 1987-04 Impact factor: 3.490

10. SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

Authors: J P Fandl; R Cabelli; D Oliver; P C Tai
Journal: Proc Natl Acad Sci U S A Date: 1988-12 Impact factor: 11.205