Literature DB >> 4560698

In vitro complementation as an assay for new proteins required for bacteriophage T4 DNA replication: purification of the complex specified by T4 genes 44 and 62.

J Barry, B Alberts.   

Abstract

We have developed an in vitro complementation assay for six T4 bacteriophage gene products believed to be components of the T4 DNA replication apparatus. This assay is based upon the fact that DNA synthesis in an infected cell lysate that lacks a given gene product is specifically stimulated by addition of the missing product. By the use of such an assay, two proteins that appear to be the products of T4 genes 44 and 62 have been purified to electrophoretic homogeneity as a single complex of the two polypeptide chains.

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Year:  1972        PMID： 4560698      PMCID： PMC427024          DOI： 10.1073/pnas.69.9.2717

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.

Authors:  H V APOSHIAN; A KORNBERG
Journal:  J Biol Chem       Date:  1962-02       Impact factor: 5.157

2.  Deoxyribonucleic acid replication in vitro.

Authors:  H Schaller; B Otto; V Nüsslein; J Huf; R Herrmann; F Bonhoeffer
Journal:  J Mol Biol       Date:  1972-01-28       Impact factor: 5.469

3.  Deoxyribonucleic acid synthesis in Escherichia coli infected with some deoxyribonucleic acid polymerase-less mutants of bacteriophage T4.

Authors:  H R Warner; J E Barnes
Journal:  Virology       Date:  1966-01       Impact factor: 3.616

4.  Coupling of late transcription to viral replication in bacteriophage T4 development.

Authors:  S Riva; A Cascino; E P Geiduschek
Journal:  J Mol Biol       Date:  1970-11-28       Impact factor: 5.469

5.  DNA synthesis in vitro.

Authors:  D W Smith; H E Schaller; F J Bonhoeffer
Journal:  Nature       Date:  1970-05-23       Impact factor: 49.962

6.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575

7.  T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.

Authors:  B M Alberts; L Frey
Journal:  Nature       Date:  1970-09-26       Impact factor: 49.962

8.  Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases.

Authors:  L M Siegel; K J Monty
Journal:  Biochim Biophys Acta       Date:  1966-02-07

9.  Properties of the DNA-delay mutants of bacteriophage T4.

Authors:  C D Yegian; M Mueller; G Selzer; V Russo; F W Stahl
Journal:  Virology       Date:  1971-12       Impact factor: 3.616

10.  RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.

Authors:  W Wickner; D Brutlag; R Schekman; A Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1972-04       Impact factor: 11.205
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  23 in total

1.  Synthesis of T4 DNA and bacteriophage in the absence of dCMP hydroxymethylase.

Authors:  D Morton; E M Kutter; B S Guttman
Journal:  J Virol       Date:  1978-10       Impact factor: 5.103

2.  Replication of T4 DNA in vitro. II. Assay system for and some properties of gene products required for T4 DNA replication.

Authors:  Y Imae; K Shinozaki; R Okazaki
Journal:  J Virol       Date:  1976-09       Impact factor: 5.103

3.  Biochemistry of DNA-defective mutants of bacteriophage T4. VI. Biological functions of gene 42.

Authors:  T W North; M E Stafford; C K Mathews
Journal:  J Virol       Date:  1976-03       Impact factor: 5.103

4.  Biochemistry of DNA-defective amber mutants of bacteriophage T4. IV. DNA synthesis in plasmolyzed cells.

Authors:  W L Collinsworth; C K Mathews
Journal:  J Virol       Date:  1974-04       Impact factor: 5.103

5.  Efficiency and frequency of translational coupling between the bacteriophage T4 clamp loader genes.

Authors:  M Y Torgov; D M Janzen; M K Reddy
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

6.  Bacteriophage T4-directed DNA synthesis in toluene-treated cells.

Authors:  Eleni Dicou; N R Cozzarelli
Journal:  J Virol       Date:  1973-12       Impact factor: 5.103

7.  Direct participation of dCMP hydroxymethylase in synthesis of bacteriophage T4 DNA.

Authors:  M G Wovcha; P K Tomich; C S Chiu; G R Greenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205

8.  Replication of bacteriophage T4 DNA in vitro. I. Basic properties of the system.

Authors:  Y Imae; R Okazaki
Journal:  J Virol       Date:  1976-08       Impact factor: 5.103

9.  Replicative bacteriophage DNA synthesis in plasmolyzed T4-infected cells: evidence for two independent pathways to DNA.

Authors:  M G Wovcha; C S Chiu; P K Tomich; G R Greenberg
Journal:  J Virol       Date:  1976-10       Impact factor: 5.103

10.  Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.

Authors:  J Hurwitz; S Wickner
Journal:  Proc Natl Acad Sci U S A       Date:  1974-01       Impact factor: 11.205
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