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Literature DB >> 6033769

The 'compartmentation' of choline acetyltransferase within the synaptosome.

Abstract

1. Choline acetyltransferase may be isolated in either a bound or soluble form after hypo-osmotic treatment of a crude synaptosome fraction, depending on the conditions. 2. In the bound form, the enzyme appears to be associated with the larger membrane fragments rather than with synaptic vesicles. 3. The bound form is predominant at slightly acid pH values and low ionic strength, the soluble form under more physiological conditions of pH and ionic strength. 4. Sodium chloride, potassium chloride, magnesium chloride and calcium chloride at similar ionic strengths solubilize the enzyme. 5. Choline acetyltransferase was found to be soluble under these conditions after release from synaptosomes from rat and pigeon cerebra, guinea-pig cortex and rabbit cortex, caudate nuclei, diencephalon and midbrain. 6. Certain isoenzymes of lactate dehydrogenase behaved similarly.

Entities: Chemical Gene Species

Mesh：

Substances：
Acyltransferases

Year: 1967 PMID： 6033769 PMCID： PMC1270396 DOI： 10.1042/bj1030262

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

25 in total

1. Intracellular distributions of acetylcholine and choline acetylase.

Authors: C O HEBB; V P WHITTAKER
Journal: J Physiol Date: 1958-06-18 Impact factor: 5.182

2. Intracellular distribution of choline acetylase.

Authors: C O HEBB; B N SMALLMAN
Journal: J Physiol Date: 1956-11-28 Impact factor: 5.182

3. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

4. Adenosinetriphosphatase activity in synaptic vesicles of rat brain.

Authors: M Germain; P Proulx
Journal: Biochem Pharmacol Date: 1965-12 Impact factor: 5.858

5. The localization of adenosine triphosphatases in morphologically characterized subcellular fractions of guinea-pig brain.

Authors: R J Hosie
Journal: Biochem J Date: 1965-08 Impact factor: 3.857

6. Species differences in subcellular distribution of choline acetylase in the CNS. A study of choline acetylase, acetylcholinesterase, 5-hydroxytryptophan decarboxylase, and monoamine oxidase in four species.

Authors: R E McCaman; G Rodríguez de Lores; E De Robertis
Journal: J Neurochem Date: 1965-11 Impact factor: 5.372

7. The separation of synaptic vesicles from nerve-ending particles ('synaptosomes').

Authors: V P Whittaker; I A Michaelson; R J Kirkland
Journal: Biochem J Date: 1964-02 Impact factor: 3.857

8. Is choline acetyltransferase present in synaptic vesicles?

Authors: F Fonnum
Journal: Biochem Pharmacol Date: 1966-10 Impact factor: 5.858

9. Inhibition of choline acetyltransferase by excess cysteine.

Authors: D Morris; C Hebb; G Bull
Journal: Nature Date: 1966-02-26 Impact factor: 49.962

10. A radiochemical method for the estimation of choline acetyltransferase.

Authors: F Fonnum
Journal: Biochem J Date: 1966-08 Impact factor: 3.857

28 in total

1. Some properties of choline acetyltransferase isolated from the electric organ ofTorpedo marmorata: Specificity for choline analogues and inhibition by certain amines and amino acids.

Authors: R R Baker; M J Dowdall
Journal: Neurochem Res Date: 1976-04 Impact factor: 3.996

2. Changes in some enzymic activities of separated neuronal and glial cell-enriched fractions from rat brains during development.

Authors: Y Nagata; T Nanba; M Ando
Journal: Neurochem Res Date: 1976-06 Impact factor: 3.996

10. The pharmacological properties of the cholinergic false transmitter, N-2-acetoxyethyl-N-methylpyrrolidinium, and its precursor, N-2-hydroxyethyl-N-methylpyrrolidinium.

Authors: I Von Schwarzenfeld; V P Whittaker
Journal: Br J Pharmacol Date: 1977-01 Impact factor: 8.739

The 'compartmentation' of choline acetyltransferase within the synaptosome.

1. Intracellular distributions of acetylcholine and choline acetylase.

2. Intracellular distribution of choline acetylase.

3. Protein measurement with the Folin phenol reagent.

4. Adenosinetriphosphatase activity in synaptic vesicles of rat brain.

5. The localization of adenosine triphosphatases in morphologically characterized subcellular fractions of guinea-pig brain.

6. Species differences in subcellular distribution of choline acetylase in the CNS. A study of choline acetylase, acetylcholinesterase, 5-hydroxytryptophan decarboxylase, and monoamine oxidase in four species.

7. The separation of synaptic vesicles from nerve-ending particles ('synaptosomes').

8. Is choline acetyltransferase present in synaptic vesicles?

9. Inhibition of choline acetyltransferase by excess cysteine.

10. A radiochemical method for the estimation of choline acetyltransferase.

1. Some properties of choline acetyltransferase isolated from the electric organ ofTorpedo marmorata: Specificity for choline analogues and inhibition by certain amines and amino acids.

2. Changes in some enzymic activities of separated neuronal and glial cell-enriched fractions from rat brains during development.

3. Studies on detergent released choline acetyltransferase from membrane fractions of rat and human brain.

4. Subcellular fractionation and distribution of cholinergic binding sites in fetal human brain.

5. The storage of transmitters in the central nervous system.

6. Synaptic transmission.

Review 7. The biochemistry of synaptic transmission.

8. Choline metabolism in the cerebral cortex of guinea pigs. Stable-bound acetylcholine.

9. Studies on the blocking action of 2-(4-phenyl piperidino) cyclohexanol (AH5183).

10. The pharmacological properties of the cholinergic false transmitter, N-2-acetoxyethyl-N-methylpyrrolidinium, and its precursor, N-2-hydroxyethyl-N-methylpyrrolidinium.