Literature DB >> 6032982

A simple method for the isolation of adrenal chromaffin granules on a large scale.

A D Smith, H Winkler.   

Abstract

Highly purified chromaffin granules can be obtained from homogenates of either ox, pig, horse or rat adrenal medullae by ultracentrifugation of the large-granule fraction layered on 1.6m-sucrose solution, by using angle-head rotors. The chromaffin granules are obtained as a pink sediment that is only slightly contaminated by mitochondria and lysosomes.

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Year:  1967        PMID： 6032982      PMCID： PMC1270431          DOI： 10.1042/bj1030480

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

1.  Improved technique for the fluorimetric estimation of catecholamines.

Authors:  U von EULER; F LISHAJKO
Journal:  Acta Physiol Scand       Date:  1961-04

2.  The distribution of the catechol amines in homogenates of the bovine adrenal medulla.

Authors:  N R EADE
Journal:  J Physiol       Date:  1958-04-03       Impact factor: 5.182

3.  Observations on the distribution of catechol amines and adenosinetriphosphate in the bovine adrenal medulla.

Authors:  H BLASCHKO; G V BORN; A D'IORIO; N R EADE
Journal:  J Physiol       Date:  1956-09-27       Impact factor: 5.182

4.  Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.

Authors:  C DE DUVE; B C PRESSMAN; R GIANETTO; R WATTIAUX; F APPELMANS
Journal:  Biochem J       Date:  1955-08       Impact factor: 3.857

5.  Determination of ATP and related compounds: firefly luminescence and other methods.

Authors:  B L STREHLER; J R TOTTER
Journal:  Methods Biochem Anal       Date:  1954

6.  Cytochrome 559 in the microsomes of the adrenal medulla.

Authors:  Y Ichikawa; T Yamano
Journal:  Biochem Biophys Res Commun       Date:  1965-07-26       Impact factor: 3.575

7.  The localization of lysosomal enzymes in chromaffin tissue.

Authors:  A D Smith; H Winkler
Journal:  J Physiol       Date:  1966-03       Impact factor: 5.182

8.  THE ADENOSINE-TRIPHOSPHATASE ACTIVITY OF ADRENAL CHROMAFFIN GRANULES.

Authors:  P BANKS
Journal:  Biochem J       Date:  1965-05       Impact factor: 3.857

9.  Purification and properties of an acidic protein from chromaffin granules of bovine adrenal medulla.

Authors:  A D Smith; H Winkler
Journal:  Biochem J       Date:  1967-05       Impact factor: 3.857

10.  Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids.

Authors:  E RACKER
Journal:  Biochim Biophys Acta       Date:  1950-01
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  80 in total

1.  Doc2 is not associated with known regulated exocytotic or endosomal compartments in adrenal chromaffin cells.

Authors:  N Charvin; G Williams; R D Burgoyne
Journal:  Biochem J       Date:  1999-07-01       Impact factor: 3.857

2.  Transgenic mouse model: a new approach for the investigation of endocrine pancreatic B-cell growth.

Authors:  R F Power; R Holm; A E Bishop; I M Varndell; S Alpert; D Hanahan; J M Polak
Journal:  Gut       Date:  1987       Impact factor: 23.059

3.  Discrimination of monoamine uptake by membranes of adrenal chromaffin granules.

Authors:  M Da Prada; R Obrist; A Pletscher
Journal:  Br J Pharmacol       Date:  1975-02       Impact factor: 8.739

4.  Glycoprotein II from adrenal chromaffin granules is also present in kidney lysosomes.

Authors:  R Weiler; H J Steiner; K W Schmid; D Obendorf; H Winkler
Journal:  Biochem J       Date:  1990-11-15       Impact factor: 3.857

5.  Lipid composition of membranes of amine-storage organelles.

Authors:  M Da Prada; A Pletscher; J P Tranzer
Journal:  Biochem J       Date:  1972-05       Impact factor: 3.857

6.  Phosphopeptidomics Reveals Differential Phosphorylation States and Novel SxE Phosphosite Motifs of Neuropeptides in Dense Core Secretory Vesicles.

Authors:  Christopher B Lietz; Thomas Toneff; Charles Mosier; Sonia Podvin; Anthony J O'Donoghue; Vivian Hook
Journal:  J Am Soc Mass Spectrom       Date:  2018-03-19       Impact factor: 3.109

7.  Exocytotic release of catecholamine from perfused adrenal gland of guinea-pig induced by veratridine.

Authors:  S Ito; Y Nakazato; A Ohga
Journal:  Br J Pharmacol       Date:  1980-12       Impact factor: 8.739

8.  Chromogranin B gene ablation reduces the catecholamine cargo and decelerates exocytosis in chromaffin secretory vesicles.

Authors:  Jésica Díaz-Vera; Yézer G Morales; Juan R Hernández-Fernaud; Marcial Camacho; Mónica S Montesinos; Federico Calegari; Wieland B Huttner; Ricardo Borges; José D Machado
Journal:  J Neurosci       Date:  2010-01-20       Impact factor: 6.167

9.  Characterization of the monoamine carrier of chromaffin granule membrane by binding of [2-3H]dihydrotetrabenazine.

Authors:  D Scherman; P Jaudon; J P Henry
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

10.  Dopamine -hydroxylase of bovine adrenal medullae. A rapid purification procedure.

Authors:  A Foldes; P L Jeffrey; B N Preston; L Austin
Journal:  Biochem J       Date:  1972-03       Impact factor: 3.857
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