Literature DB >> 6021045

Artifact produced in disc electrophoresis by ammonium persulfate.

J M Brewer.   

Abstract

Ammonium persulfate, a common polymerizing agent for acrylamide gels, can inactivate yeast enolase and produce increased electrophoretic heterogeneity during disc electrophoresis in gels containing 8M urea. The use of riboflavin and light for polymerization or thioglycolate for removal of the persulfate are feasible alternatives.

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Year:  1967        PMID： 6021045     DOI： 10.1126/science.156.3772.256

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  16 in total

1.  Plant Carbonic Anhydrases: I. Distribution of Types among Species.

Authors:  C A Atkins; B D Patterson; D Graham
Journal:  Plant Physiol       Date:  1972-08       Impact factor: 8.340

2.  Specific dimerization of the light chains of human immunoglobulin.

Authors:  G T Stevenson; D Straus
Journal:  Biochem J       Date:  1968-07       Impact factor: 3.857

3.  Horizontal polyacrylamide electrophoresis for the determination of serum protein (haptoglobin) and red cell enzyme polymorphisms.

Authors:  H H Hoppe; W Hennig; B Brinkmann
Journal:  Humangenetik       Date:  1972

4.  Chromatographic evidence for the existence of multiple forms of cathepsin B1.

Authors:  S G Franklin; R M Metrione
Journal:  Biochem J       Date:  1972-03       Impact factor: 3.857

5.  An electrophoretic study of the low-molecular-weight components of myosin.

Authors:  W T Perrie; S V Perry
Journal:  Biochem J       Date:  1970-08       Impact factor: 3.857

6.  The composition of rat liver microsomes. The structural proteins of rat liver microsomes.

Authors:  K A Ward; J K Pollak
Journal:  Biochem J       Date:  1969-08       Impact factor: 3.857

7.  Nonidentical subunits of bacterial luciferase: their isolation and recombination to form active enzyme.

Authors:  J Friedland; J W Hastings
Journal:  Proc Natl Acad Sci U S A       Date:  1967-12       Impact factor: 11.205

8.  Rapid electrotransfer probing for improved detection sensitivity in in-gel immunoassays.

Authors:  Andoni P Mourdoukoutas; Samantha M Grist; Amy E Herr
Journal:  Anal Methods       Date:  2020-10-08       Impact factor: 2.896

9.  Tentative identification of a vasopressin-neurophysin and an oxytocin-neurophysin in the rat.

Authors:  G D Burford; C W Jones; B T Pickering
Journal:  Biochem J       Date:  1971-10       Impact factor: 3.857

10.  Occurrence and properties of lactic dehydrogenases of fermentative mycoplasmas.

Authors:  H Neimark; R M Lemcke
Journal:  J Bacteriol       Date:  1972-09       Impact factor: 3.490
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