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Literature DB >> 600269

Carbohydrate composition of purified human liver alpha-L-fucosidase.

Abstract

Human liver alpha-L-fucosidase was purified to apparent homogeneity and analyzed for carbohydrate content primarily by gas-liquid chromatography (glc). The enzyme is about 7% carbohydrate by weight and contains the following sugars (residues per 50, 000 molecular weight subunit): mannose (8.3), glucosamine (4.3) (presumably N-acetylated), sialic acid (1.6) and glucose (1.6). Galactose (0.8) and L-fucose (1.8) were also found but their presence may be due to artifacts of the purification procedure.

Entities: Chemical Species

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Year: 1977 PMID： 600269 DOI： 10.1007/bf00215277

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

18 in total

1. Lysosomal hydrolases: Conversion of acidic to basic forms by neuraminidase.

Authors: A Goldstone; P Konecny; H Koenig
Journal: FEBS Lett Date: 1971-02-12 Impact factor: 4.124

2. Polymorphism of human alpha fucosidase.

Authors: B M Turner; V S Turner; N G Beratis; K Hirschhorn
Journal: Am J Hum Genet Date: 1975-09 Impact factor: 11.025

3. Isoelectric focusing of isoenzymes of human liver alpha-L-fucosidase.

Authors: R Thorpe; D Robinson
Journal: FEBS Lett Date: 1975-06-01 Impact factor: 4.124

4. Recognition of lysosomal glycosidases in vivo inhibited by modified glycoproteins.

Authors: P Stahl; P H Schlesinger; J S Rodman; T Doebber
Journal: Nature Date: 1976-11-04 Impact factor: 49.962

5. The preparation and chemical composition of the multiple forms of beta-glucuronidase from the female rat preputial gland.

Authors: D R Tulsiani; R K Keller; O Touster
Journal: J Biol Chem Date: 1975-06-25 Impact factor: 5.157

6. Human liver alpha-L-fucosidase. Purification, characterization, and immunochemical studies.

Authors: J A Alhadeff; A L Miller; H Wenaas; T Vedvick; J S O'Brien
Journal: J Biol Chem Date: 1975-09-25 Impact factor: 5.157

7. The abnormalities of lysosomal enzymes in mucopolysacc- haridoses.

Authors: F Van Hoof; H G Hers
Journal: Eur J Biochem Date: 1968-12

8. On the existence and the enzymic interconversion of the isozymes of alpha-galactosidase in human organs.

Authors: J A Kint
Journal: Arch Int Physiol Biochim Date: 1971-08

Review 9. Comparative aspects of glycoprotein structure.

Authors: R Kornfeld; S Kornfeld
Journal: Annu Rev Biochem Date: 1976 Impact factor: 23.643

10. The purification and characterization of rat liver lysosomal alpha-L-fucosidase.

Authors: D J Opheim; O Touster
Journal: J Biol Chem Date: 1977-01-25 Impact factor: 5.157

7 in total

1. Isoenzymes of human liver alpha-L-fucosidase: chemical relationship, kinetic studies, and immunochemical characterization.

Authors: J A Alhadeff; G Cimino; A Janowsky
Journal: Mol Cell Biochem Date: 1978-05-31 Impact factor: 3.396

7. Radioimmunochemical evidence for a role of carbohydrate in antigenic determinant(s) on human liver alpha-L-fucosidase.

Authors: J A Alhadeff; G L Andrews-Smith
Journal: Biochem J Date: 1984-10-15 Impact factor: 3.857