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Literature DB >> 5969283

The active centre of triose phosphate isomerase.

Abstract

The molecular weight and amino acid composition of triose phosphate isomerase have been determined. The molecular weight (43000) is lower and the molecular activity (500000) higher than those of most other glycolytic enzymes. Reaction with iodoacetate (studied with radioactive reagent) takes place in two phases: in the first phase, at pH6.3, cysteine and methionine groups react and enzymic activity is unimpaired; in the second phase, histidine reacts and enzymic activity is lost. Photo-oxidation leads to inactivation, with loss of cysteine, of histidine and of tryptophan, but little loss of tyrosine. The mechanism postulated for the action of the enzyme demands the intervention of a group functioning as a base, and the results obtained are consistent with histidine's being the basic group in the active centre.

Entities: Chemical Gene

Mesh：

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Year: 1966 PMID： 5969283 PMCID： PMC1265204 DOI： 10.1042/bj1000702

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

26 in total

1. The reaction of iodoacetate with methionine.

Authors: H G GUNDLACH; S MOORE; W H STEIN
Journal: J Biol Chem Date: 1959-07 Impact factor: 5.157

2. THE REACTIVITIES OF THE HISTIDINE RESIDUES AT THE ACTIVE SITE OF RIBONUCLEASE TOWARD HALO ACIDS OF DIFFERENT STRUCTURES.

Authors: R L HEINRIKSON; W H STEIN; A M CRESTFIELD; S MOORE
Journal: J Biol Chem Date: 1965-07 Impact factor: 5.157

3. Tetrose diphosphate, a specific inhibitor of glyceraldehyde 3-phosphate dehydrogenase.

Authors: E RACKER; V KLYBAS; M SCHRAMM
Journal: J Biol Chem Date: 1959-10 Impact factor: 5.157

4. [Quantitative determination of amino acid composition of protein hydrolysates by combined electrophoresis and chromatography].

Authors: W GRASSMANN; K HANNIG; M PLOCKL
Journal: Hoppe Seylers Z Physiol Chem Date: 1955

5. [Simplification and improvement in the method for the quantitative determination of amino acids and peptides by means of ninhydrin-copper complexes].

Authors: F BODE
Journal: Biochem Z Date: 1955

2. Isoenzymes of Glutamine Synthetase in Roots of Pea (Pisum sativum L. cv Little Marvel) and Alfalfa (Medicago media Pers. cv Saranac).

Authors: L P Vézina; H J Hope; K W Joy
Journal: Plant Physiol Date: 1987-01 Impact factor: 8.340

7. Methods for starch-gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscles of a variety of species.

Authors: R K Scopes
Journal: Biochem J Date: 1968-03 Impact factor: 3.857

The active centre of triose phosphate isomerase.

1. The reaction of iodoacetate with methionine.

2. THE REACTIVITIES OF THE HISTIDINE RESIDUES AT THE ACTIVE SITE OF RIBONUCLEASE TOWARD HALO ACIDS OF DIFFERENT STRUCTURES.

3. Tetrose diphosphate, a specific inhibitor of glyceraldehyde 3-phosphate dehydrogenase.

4. [Quantitative determination of amino acid composition of protein hydrolysates by combined electrophoresis and chromatography].

5. [Simplification and improvement in the method for the quantitative determination of amino acids and peptides by means of ninhydrin-copper complexes].

6. The action of trypsin on polylysine.

7. Intramolecular hydrogen transfer in the phosphoglucose isomerase reaction.

8. Degradation, structure and some derivatives of cephalosporin N.

9. The thiol groups of yeast alcohol dehydrogenase.

10. Characterization of a human macroglobulin. II. Distribution of the disulfide bonds.

1. Studies on the sub-units of triose phosphate isomerase.

2. Isoenzymes of Glutamine Synthetase in Roots of Pea (Pisum sativum L. cv Little Marvel) and Alfalfa (Medicago media Pers. cv Saranac).

3. Glycolytic enzymes as a multi-enzyme system.

4. The pK of the carboxyl group at the active centre of triose phosphate isomerase.

5. Amino acid sequences around the cysteine residues of rabbit muscle triose phosphate isomerase.

6. Disequilibrium in the triose phosphate isomerase system in rat liver.

7. Methods for starch-gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscles of a variety of species.

8. A reappraisal of some structural features of bovine heart malate dehydrogenase.

9. Purification and Properties of Glutamine Synthetase in Leaves and Roots of Pinus banksiana Lamb.

10. The active centre of rabbit muscle triose phosphate isomerase. The site that is labelled by glycidol phosphate.