Literature DB >> 5968977

Isolation and fractionation of rat brain nuclei.

H Lovtrup-Rein, B S McEwen.   

Abstract

A method for isolating pure and unaltered nuclei from rat brain by means of differential centrifugation is described. The isolated nuclei are further separated into discrete fractions of neuronal, astrocytic, and glial nuclei, with a yield amounting to 20 to 25% of the DNA of the original homogenate. Both the morphology and size of the nuclei remained unchanged. Problems concerning the composition of the isolation media, the use of detergents, as well as those raised by density gradient centrifugation in sucrose, Ficoll, and Dextran are discussed. Some values for the density of each type of brain nuclei are suggested.

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Year:  1966        PMID： 5968977      PMCID： PMC2107004          DOI： 10.1083/jcb.30.2.405

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  12 in total

1.  ISOLATION OF NUCLEI FROM MAMMALIAN TISSUES THROUGH THE USE OF TRITON X-100.

Authors:  W C HYMER; E L KUFF
Journal:  J Histochem Cytochem       Date:  1964-05       Impact factor: 2.479

2.  A density gradient for the isolation of metabolically active thymus nuclei.

Authors:  W D FISHER; G B CLINE
Journal:  Biochim Biophys Acta       Date:  1963-04-30

3.  Biochemistry of the developing rat brain. I. Soluble enzymes in isolated neonatal brain nuclei.

Authors:  D A RAPPOPORT; R R FRITZ
Journal:  Biochim Biophys Acta       Date:  1963-07-02

4.  Fractionation of rat liver nuclei into diploid and tetraploid DNA classes by continuous density gradient sedimentation.

Authors:  J A FALZONE; C H BARROWS; M J YIENGST
Journal:  Exp Cell Res       Date:  1962-03       Impact factor: 3.905

5.  Possible use of synthetic surface-active agents for the preparation of tumour cell nuclei.

Authors:  M E HODES; C G PALMER; D S WILLIAMS
Journal:  Nature       Date:  1958-08-23       Impact factor: 49.962

6.  Isolation of pure and unaltered liver nuclei morphology and biochemical composition.

Authors:  J CHAUVEAU; Y MOULE; C ROUILLER
Journal:  Exp Cell Res       Date:  1956-08       Impact factor: 3.905

7.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

8.  Isolated nuclei from cells of the cerebral cortex; preparation and enzyme content.

Authors:  D RICHTER; R P HULLIN
Journal:  Biochem J       Date:  1951-04       Impact factor: 3.857

9.  Isolation and some characteristics of cell nuclei from brain cortex of adult cat.

Authors:  A A Hadjiolov; Z S Tencheva; A G Bojadjieva-Mikhailova
Journal:  J Cell Biol       Date:  1965-08       Impact factor: 10.539

10.  The isolation of cell nuclei from rat brain.

Authors:  M B SPORN; T WANKO; W DINGMAN
Journal:  J Cell Biol       Date:  1962-10       Impact factor: 10.539
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  35 in total

1.  Identification of long-lived proteins reveals exceptional stability of essential cellular structures.

Authors:  Brandon H Toyama; Jeffrey N Savas; Sung Kyu Park; Michael S Harris; Nicholas T Ingolia; John R Yates; Martin W Hetzer
Journal:  Cell       Date:  2013-08-29       Impact factor: 41.582

2.  Mass isolation of pea nuclei.

Authors:  K J Tautvydas
Journal:  Plant Physiol       Date:  1971-04       Impact factor: 8.340

3.  Nucleo-cytoplasmic relationships of high-molecular-weight ribonucleic acid, including polyadenylated species, in the developing rat brain.

Authors:  W Berthold; L Lim
Journal:  Biochem J       Date:  1976-02-15       Impact factor: 3.857

4.  Increased nuclear permeability is a driver for age-related motoneuron loss.

Authors:  Ashley Gillon; Charlotte Steel; Jon Cornwall; Philip Sheard
Journal:  Geroscience       Date:  2020-01-30       Impact factor: 7.713

5.  Effect of a brain-specific protein (S-100 protein) on the nucleolar RNA polymerase activity in isolated brain nuclei.

Authors:  N Miani; F Michetti; G De Renzis; A Caniglia
Journal:  Experientia       Date:  1973-12

6.  Fractionation and characterization of cell nuclei from rat neocortex by protein-DNA ratios.

Authors:  H R Olpe; H P von Hahn; C G Honegger
Journal:  Experientia       Date:  1972-07-15

7.  Fractionation of nuclei from brain by zonal centrifugation and a study of the ribonucleic acid polymerase activity in the various classes of nuclei.

Authors:  J Austoker; D Cox; A P Mathias
Journal:  Biochem J       Date:  1972-10       Impact factor: 3.857

8.  The activity of deoxyribonucleic acid polymerase and deoxyribonucleic acid synthesis in nuclei from brain fractionated by zonal centrifugation.

Authors:  M A Stambolova; D Cox; A P Mathias
Journal:  Biochem J       Date:  1973-11       Impact factor: 3.857

9.  Uptake of oestradiol by the rabbit hypothalamus. Specificity of binding by nuclei in vitro.

Authors:  G J Chader; C A Villee
Journal:  Biochem J       Date:  1970-06       Impact factor: 3.857

10.  Validation of the isotropic fractionator: comparison with unbiased stereology and DNA extraction for quantification of glial cells.

Authors:  Jami Bahney; Christopher S von Bartheld
Journal:  J Neurosci Methods       Date:  2013-11-12       Impact factor: 2.390
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