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Literature DB >> 5965342

The enzymic estimation of glutamate and glutamine.

Abstract

A method of estimating glutamic acid is described, based on its dehydrogenation by glutamate dehydrogenase coupled, by means of N-methylphenazine methosulphate, to the reduction of tetrazolium salts. The method is suitable for the estimation of 0-0.3mumole of glutamic acid. The response is linear, but not stoicheiometric: possible reasons for this are discussed. If suitable precautions are taken, the use of a partially purified preparation of glutaminase makes it possible to estimate glutamine also.

Entities: Chemical

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Year: 1966 PMID： 5965342 PMCID： PMC1264982 DOI： 10.1042/bj0990246

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

13 in total

9. [Determination of L-lactate, L-malate, L-glutamate and ethyl alcohol in the enzyme-optic test by means of the DPN analog, 3-acetylpyridine-DPN].

Authors: H HOLZER; H D SOELING
Journal: Biochem Z Date: 1962

10. Some rate constants for the phenazine methosulphate-catalysed oxidation of reduced nicotinamide-adenine dinucleotide.

Authors: J H Ottaway
Journal: Biochem J Date: 1966-04 Impact factor: 3.857

4 in total

The enzymic estimation of glutamate and glutamine.

1. THE NATURE OF THE RADIATION-INDUCED LESION OF THE ELECTRON TRANSPORT CHAIN OF THYMUS MITOCHONDRIA.

2. Studies on the succinate-neotetrazolium reductase system. Activation by vitamin K3.

3. Sites of electron transfer to tetrazolium salts in the succinoxidase system.

4. Glutamic dehydrogenase. I. The effect of coenzyme on the sedimentation velocity and kinetic behavior.

5. A colorimetric method for the assay of soluble succinic dehydrogenase and pyridinenucleotide-linked dehydrogenases.

6. Zinc in beef liver glutamic dehydrogenase.

7. Partial purification and properties of renal glutaminase.

8. Kinetic and equilibrium studies on crystalline 1-glutamic acid dehydrogenase.

9. [Determination of L-lactate, L-malate, L-glutamate and ethyl alcohol in the enzyme-optic test by means of the DPN analog, 3-acetylpyridine-DPN].

10. Some rate constants for the phenazine methosulphate-catalysed oxidation of reduced nicotinamide-adenine dinucleotide.

1. The concentration of asparagine in the tissues of rats treated with growth hormone.

2. Metabolism, sperm and fluid production of the isolated perfused testis of the sheep and goat.

3. Some rate constants for the phenazine methosulphate-catalysed oxidation of reduced nicotinamide-adenine dinucleotide.

4. Quantification of Glutamate and Aspartate by Ultra-High Performance Liquid Chromatography.