Some rate constants for the phenazine methosulphate-catalysed oxidation of reduced nicotinamide-adenine dinucleotide.

Kinetic measurements have been made on the phenazine methosulphate-catalysed oxidation of NADH(2) and the effects of cyanide, EDTA, Fe(2+), catalase and an unidentified mitochondrial factor on this reaction are examined. Chemical equations are formulated and values of the rate constants are determined. The data suggest the existence of an unreactive form of the reduced phenazine methosulphate; the stability and possible nature of such a compound are discussed.