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Literature DB >> 5949569

Purification of beta-acetylglucosaminase and beta-galactosidase from ram testis.

Abstract

1. The presence of beta-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. 2. The purification of beta-acetylglucosaminase (EC 3.2.1.30) and of beta-galactosidase from the ram-testis extract by ammonium sulphate precipitation and chromatography on a CM-cellulose column is described. 3. The final purifications of the separated enzymes achieved were for the beta-acetylglucosaminase 35 times and for the beta-galactosidase 99 times. 4. The possibility of using DEAE-cellulose and Sephadex G-200 to purify the enzymes was investigated.

Entities: Chemical Gene

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Year: 1966 PMID： 5949569 PMCID： PMC1264859 DOI： 10.1042/bj0980405

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

8 in total

7. [Studies on the linkage of carbohydrate to peptide in ovomucoid. I. Carbohydrate fragment obtained by baryta-hydrolysis of ovomucoid].

Authors: M TANAKA
Journal: Yakugaku Zasshi Date: 1961-10 Impact factor: 0.302

8. beta-D-Fucosidase in the Limpet, Patella vulgata.

Authors: G A LEVVY; A McALLAN
Journal: Biochem J Date: 1963-04 Impact factor: 3.857

8 in total

7 in total

Purification of beta-acetylglucosaminase and beta-galactosidase from ram testis.

1. [Studies on the linkage of carbohydrate to peptide in ovomucoid. II. Mucopeptides obtained by pancreatic digestion].

2. The attachment of carbohydrate in ovomucoid.

3. Studies on glucosaminidase. 3. Testicular N-acetyl-beta-glucosaminidase and N-acetyl-beta-galactosaminidase.

4. Purification of beta-N-acetylglucosaminidase from the pig epididymis.

5. Mammalian glycosidases. 2. Properties of alpha-mannosidase and beta-galactosidase from rat epididymis.

6. The beta-d-galactosidase of Escherichia coli, strain K-12.

7. [Studies on the linkage of carbohydrate to peptide in ovomucoid. I. Carbohydrate fragment obtained by baryta-hydrolysis of ovomucoid].

8. beta-D-Fucosidase in the Limpet, Patella vulgata.

1. Some properties of pig epididymal beta-N-acetyl-D-glucosaminidase.

2. Absence of -N-acetyl-D-hexosaminidase A activity in a healthy woman.

3. Comparison of the N-acetylhexosaminidase components in the ram testis and epididymis by isoelectric focusing.

4. The N-acetylhexosaminidase components of the ram testis and epididymis.

5. N-Acetyl-beta-glucosaminidases in human spleen.

6. Enzymes of Trichomonas foetus. Separation and properties of two beta-galactosidases.

7. Glycosidases in normal and dimethylhydrazine-treated rats and mice with special reference to the colonic tumours.