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Literature DB >> 4733242

The N-acetylhexosaminidase components of the ram testis and epididymis.

Abstract

Three and four N-acetylhexosaminidase components, from ram testis and epididymis respectively, have been separated by ion-exchange chromatography on DEAE-cellulose. Although they all have the same molecular weight (approx. 140000) and very similar catalytic properties towards the synthetic substrates, 4-methylumbelliferyl N-acetyl-beta-glucosaminide and N-acetyl-beta-galactosaminide, isoelectric focusing of the individual components showed that each had a distinct pI value. Isoelectric focusing has also been used to demonstrate the occurrence of multiple forms in ejaculated ram semen.

Entities: Chemical Disease

Mesh：

Substances：
Hexosaminidases

Year: 1973 PMID： 4733242 PMCID： PMC1177738 DOI： 10.1042/bj1330593

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

16 in total

7. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.

Authors: O Vesterberg; H Svensson
Journal: Acta Chem Scand Date: 1966

2. Kinetics and thermodynamic transitions of N-acetyl-beta-D-glucosaminidase A and B in free and bound forms: role of cellulose ion-exchangers.

Authors: G S Gupta; R Raina
Journal: Mol Cell Biochem Date: 1989-03-16 Impact factor: 3.396

2 in total

The N-acetylhexosaminidase components of the ram testis and epididymis.

1. Glycosidases in mammalian sperm and seminal plasma.

2. Protein measurement with the Folin phenol reagent.

3. Some properties of pig epididymal beta-N-acetyl-D-glucosaminidase.

Review 4. Estimation of molecular size and molecular weights of biological compounds by gel filtration.

5. [Molecular forms of N-acetyl- -D-glucosaminidase from boar epididymis].

6. Differential assay of human hexosaminidases A and B.

7. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.

8. Purification of beta-acetylglucosaminase and beta-galactosidase from ram testis.

9. Comparison of the N-acetylhexosaminidase components in the ram testis and epididymis by isoelectric focusing.

10. N-Acetyl-beta-glucosaminidases in human spleen.

1. Purification of N-acetyl-hexosaminidase from boar epididymis by affinity chromatography.

2. Kinetics and thermodynamic transitions of N-acetyl-beta-D-glucosaminidase A and B in free and bound forms: role of cellulose ion-exchangers.