Literature DB >> 59420

A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.

R J Wassersug.   

Abstract

This paper describes a modification of the Simons and Van Horn (1971) procedure for rendering cartilage blue, bone red, and soft tissue translucent or transparent in whole vertebrate specimens. Alcian blue and alizarin red S are used to stain cartilage and bone respectively. In our procedure formalin is used as a fixative. This is a significant modification because formalin is the common fixative for museum specimens. This clearing and staining procedure is thus readily applicable to comparative studies in anatomy, embryology and systematic zoology.

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Year:  1976        PMID： 59420     DOI： 10.3109/10520297609116684

Source DB:  PubMed          Journal:  Stain Technol        ISSN： 0038-9153


  40 in total

1.  Growth of embryonic chick tibiae in ovo and in vitro: a scanning electron microscope study.

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2.  Interference with interparietal growth in the human skull by the tectum synoticum posterior.

Authors:  S Niida; A Yamasaki; H Kodama
Journal:  J Anat       Date:  1992-02       Impact factor: 2.610

3.  Development of the pelvis and posterior part of the vertebral column in the Anura.

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Journal:  J Anat       Date:  2005-01       Impact factor: 2.610

4.  Correlation of the shoulder girdle asymmetry with the limb skeleton asymmetry in Xenopus laevis.

Authors:  M A Kostylev; Y B Malashichev
Journal:  Dokl Biol Sci       Date:  2007 Sep-Oct

5.  Maternal genetic effects in Astyanax cavefish development.

Authors:  Li Ma; Allen G Strickler; Amy Parkhurst; Masato Yoshizawa; Janet Shi; William R Jeffery
Journal:  Dev Biol       Date:  2018-07-19       Impact factor: 3.582

6.  The unusual orbitosphenoid of the snakelike lizard Bachia bicolor.

Authors:  Oscar A Tarazona; Martha Patricia Ramírez-Pinilla
Journal:  J Anat       Date:  2008-08       Impact factor: 2.610

7.  Occipital roof development in the Japanese musk shrew, Suncus murinus.

Authors:  S Niida; N Okada; H Wakisaka; K Miyata; N Maeda
Journal:  J Anat       Date:  1994-10       Impact factor: 2.610

8.  Dissecting the genetic complexity of human 6p deletion syndromes by using a region-specific, phenotype-driven mouse screen.

Authors:  Debora Bogani; Catherine Willoughby; Jennifer Davies; Kulvinder Kaur; Ghazala Mirza; Anju Paudyal; Heather Haines; Richard McKeone; Matthew Cadman; Guido Pieles; Jürgen E Schneider; Shoumo Bhattacharya; Andrea Hardy; Patrick M Nolan; Nikos Tripodis; Michael J Depew; Ramya Chandrasekara; Gimara Duncan; Paul T Sharpe; Andy Greenfield; Paul Denny; Steve D M Brown; Jiannis Ragoussis; Ruth M Arkell
Journal:  Proc Natl Acad Sci U S A       Date:  2005-08-18       Impact factor: 11.205

9.  Effect of bone morphogenetic protein signaling on development of the jaw skeleton.

Authors:  Diane Hu; Celine Colnot; Ralph S Marcucio
Journal:  Dev Dyn       Date:  2008-12       Impact factor: 3.780

10.  The genesis of cartilage size and shape during development and evolution.

Authors:  B Frank Eames; Richard A Schneider
Journal:  Development       Date:  2008-10-30       Impact factor: 6.868
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