| Literature DB >> 594188 |
M Hillar, S A Davis, D Chambers.
Abstract
A heterogeneous poly A-mRNA fraction was isolated from rat liver microsomes by phenol:chloroform extraction, millipore filtration, and poly U-agarose affinity chromatography. The fractions were characterized by their secondary structures and poly A contents. From translational studies, the isolated fraction was found to have high glutamate dehydrogenase template activity in cell-free systems containing microsomes or polysomes. A spectrophotometric procedure for following enzyme biosynthesis was also developed.Entities:
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Year: 1977 PMID： 594188
Source DB: PubMed Journal: Physiol Chem Phys ISSN： 0031-9325