Literature DB >> 2876375

Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe.

M Hillar, S Orudiakomo.   

Abstract

Specific messenger RNA for glutamate dehydrogenase was partially purified from a calf liver polysomal poly(A)-mRNA fraction by sucrose density gradient centrifugation. Enzyme activity in the translational incubation mixture was detected by measuring NADH oxidation in the presence of alpha-ketoglutarate and ammonia as a decrease in absorbency 340-442 nm in a dual wavelength Aminco DW-2 spectrophotometer.

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Year:  1986        PMID： 2876375     DOI： 10.1007/bf00419732

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  9 in total

1.  Messenger RNA in undeveloped and developing Artemia salina embryos.

Authors:  J M Sierra; W Filipowicz; S Ochoa
Journal:  Biochem Biophys Res Commun       Date:  1976-03-08       Impact factor: 3.575

2.  Use of antibodies for the isolation of biologically pure messenger ribonucleic acid from fully functional eukaryotic cells.

Authors:  I Schechter
Journal:  Biochemistry       Date:  1974-04-23       Impact factor: 3.162

3.  Glutamate dehydrogenase activity in subcellular fractions of mouse fibrosarcoma.

Authors:  M Hillar; B Lennox
Journal:  Mol Biol Rep       Date:  1979-12-31       Impact factor: 2.316

4.  An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors:  H R Pelham; R J Jackson
Journal:  Eur J Biochem       Date:  1976-08-01

5.  Translation of glutamate dehydrogenase mRNA in a reticulocyte cell-free system.

Authors:  M Hillar; W Makarewicz
Journal:  Physiol Chem Phys       Date:  1979

6.  Studies on a rat liver subcellular poly A-rich RNA fraction with glutamate dehydrogenase template activity.

Authors:  M Hillar; S A Davis; D Chambers
Journal:  Physiol Chem Phys       Date:  1977

7.  Hormonal induction of glutamate dehydrogenase in rat liver.

Authors:  M Hillar; J Reuben
Journal:  Experientia       Date:  1976-05-15

8.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

9.  Spectra of glutamate dehydrogenase with diethylstilbestrol.

Authors:  M Hillar
Journal:  Arch Int Physiol Biochim       Date:  1978-02
  9 in total

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