Transport and metabolism of fatty acids by isolated rumen epithelium.

1. The metabolism of even-numbered saturated (acetic acid to stearic acid) and unsaturated (oleic acid and linolenic acid) fatty acids by diaphragms of isolated rumen epithelium has been investigated. 2. When fatty acids are presented to the papillae surface, ketone bodies are released from the opposite (muscle) side of the tissue. 3. When the concentration of octanoate or decanoate is increased to a critical value, which varies inversely with the chain length of the fatty acid, the respiration of the tissue is inhibited and ketone body synthesis is diminished. Under these conditions unmetabolized fatty acid crosses the tissue down a concentration gradient. 4. The inhibitions by octanoate and decanoate are more marked when the fatty acid is presented to both surfaces of the rumen epithelium. 5. During the oxidation of octanoate and decanoate at non-inhibitory concentrations, small quantities of shorter chain fatty acids, including acetate, are produced.