Literature DB >> 593881

Interactions of norharman and harman with DNA.

K Hayashi, M Nagao, T Sugimura.   

Abstract

The interactions of norharman (9H-pyrido [3,4-b] indole) and harman (1-methyl-9H-pyrido [3,4-b] indole) with DNA were studied. DNA caused remarkable fluorescence quenching and change in the absorption spectra of the dyes. Scatchard plots obtained by optical titration gave Kd values of 2.2 X 10(-5)M and 7.7 X 10(-6)M, and apparent numbers of binding sites of 0.13/base and 0.12/base for norharman and harman, respectively. Agarose gel electrophoresis of circular DNA, closed in the presence or absence of norharman revealed that the dye intercalates DNA, thereby causing 17 +/- 3 degrees unwinding of the double helix.

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Year:  1977        PMID： 593881      PMCID： PMC343192          DOI： 10.1093/nar/4.11.3679

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  5 in total

1.  Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers.

Authors:  D E Pulleyblank; M Shure; D Tang; J Vinograd; H P Vosberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

2.  Conformational fluctuations of DNA helix.

Authors:  D E Depew; J C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

3.  The number of superhelical turns in native virion SV40 DNA and minicol DNA determined by the band counting method.

Authors:  M Shure; J Vinograd
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

4.  The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.

Authors:  J C Wang
Journal:  J Mol Biol       Date:  1974-11-15       Impact factor: 5.469

5.  Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.

Authors:  W Keller
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205
  5 in total
  9 in total

1.  Synthesis of the carboline disaccharide domain of shishijimicin A.

Authors:  K C Nicolaou; J L Kiappes; Weiwei Tian; Vijaya B Gondi; Jochen Becker
Journal:  Org Lett       Date:  2011-06-28       Impact factor: 6.005

2.  Harmines inhibit cancer cell growth through coordinated activation of apoptosis and inhibition of autophagy.

Authors:  Xinran Geng; Yichang Ren; Fangfang Wang; Danmei Tian; Xinsheng Yao; Youwei Zhang; Jinshan Tang
Journal:  Biochem Biophys Res Commun       Date:  2018-03-01       Impact factor: 3.575

3.  DNA-binding studies of the natural β-carboline eudistomin U.

Authors:  Jennifer M Giulietti; Patrick M Tate; Ang Cai; Bongsup Cho; Seann P Mulcahy
Journal:  Bioorg Med Chem Lett       Date:  2016-08-18       Impact factor: 2.823

4.  Comutagenic effect of norharman and harman with 2-acetylaminofluorene derivatives.

Authors:  K Umezawa; A Shirai; T Matsushima; T Sugimura
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

5.  Selective inhibition by harmane of the apurinic apyrimidinic endonuclease activity of phage T4-induced UV endonuclease.

Authors:  H R Warner; M L Persson; R J Bensen; D W Mosbaugh; S Linn
Journal:  Nucleic Acids Res       Date:  1981-11-25       Impact factor: 16.971

6.  There is a correlation between the DNA affinity and mutagenicity of several 3-amino-1-methyl-5H-pyrido[4,3-b]indoles.

Authors:  J M Pezzuto; P P Lau; Y Luh; P D Moore; G N Wogan; S M Hecht
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

7.  Synthesis and In Vitro Antitumor Activity of Novel Bivalent β-Carboline-3-carboxylic Acid Derivatives with DNA as a Potential Target.

Authors:  Hongling Gu; Na Li; Jiangkun Dai; Yaxi Xi; Shijun Wang; Junru Wang
Journal:  Int J Mol Sci       Date:  2018-10-15       Impact factor: 5.923

8.  Computational Insights into β-Carboline Inhibition of Monoamine Oxidase A.

Authors:  Alja Prah; Tanja Gavranić; Andrej Perdih; Marija Sollner Dolenc; Janez Mavri
Journal:  Molecules       Date:  2022-10-09       Impact factor: 4.927

9.  Sequence specific binding of beta carboline alkaloid harmalol with deoxyribonucleotides: binding heterogeneity, conformational, thermodynamic and cytotoxic aspects.

Authors:  Sarita Sarkar; Prateek Pandya; Kakali Bhadra
Journal:  PLoS One       Date:  2014-09-23       Impact factor: 3.240
  9 in total

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