Literature DB >> 5938667

Selective degradation of thymidine and thymine deoxynucleotides.

K Burton, W T Riley.   

Abstract

1. Osmium tetroxide in dilute ammonia oxidizes various pyrimidine nucleosides at different rates. Thymidine is oxidized about 45 times as fast as deoxycytidine. The phosphate groups may be eliminated from oxidized thymine nucleotides by successive treatments with alkali and then with diphenylamine in aqueous formic acid. The reactions can be applied to the selective degradation of thymidine in oligodeoxynucleotides.

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Year:  1966        PMID： 5938667      PMCID： PMC1264796          DOI： 10.1042/bj0980070

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  NUCLEOTIDE SEQUENCE IN DEOXYRIBONUCLEIC ACIDS: THE DETERMINATION OF THE DISTRIBUTION OF ADENINE RESIDUES.

Authors:  A S JONES; R T WALKER
Journal:  Nature       Date:  1964-04-04       Impact factor: 49.962

2.  THE DYE-SENSITIZED PHOTOOXIDATION OF PURINE AND PYRIMIDINE DERIVATIVES.

Authors:  M I SIMON; H VANVUNAKIS
Journal:  Arch Biochem Biophys       Date:  1964-04       Impact factor: 4.013

3.  The distribution of nucleotides in deoxyribonucleic acid.

Authors:  G B PETERSEN
Journal:  Biochem J       Date:  1963-06       Impact factor: 3.857

4.  The structure of the DNA of bacteriophage x-174. IV. Pyrimidine sequences.

Authors:  J B HALL; R L SINSHEIMER
Journal:  J Mol Biol       Date:  1963-02       Impact factor: 5.469

5.  A colorimetric determination of purine and pyrimidine deoxyribonucleosides and their phosphorylated derivatives.

Authors:  L GROSSMAN; J GREENLEES
Journal:  Anal Biochem       Date:  1961-06       Impact factor: 3.365

6.  The frequencies of certain sequences of nucleotides in deoxyribonucleic acid.

Authors:  K BURTON; G B PETERSEN
Journal:  Biochem J       Date:  1960-04       Impact factor: 3.857

7.  The fluorometric determination of thymine in deoxyribonucleic acid and derivatives.

Authors:  D ROBERTS; M FRIEDKIN
Journal:  J Biol Chem       Date:  1958-08       Impact factor: 5.157

8.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

9.  The large scale preparation of sodium desoxyribonucleate from ripe salmon testes.

Authors:  C F EMANUEL; I L CHAIKOFF
Journal:  J Biol Chem       Date:  1953-07       Impact factor: 5.157

10.  An improved method for the colorimetric determination of phosphate.

Authors:  I Berenblum; E Chain
Journal:  Biochem J       Date:  1938-02       Impact factor: 3.857
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  23 in total

1.  cDNA cloning, expression and functional characterization of an Arabidopsis thaliana homologue of the Escherichia coli DNA repair enzyme endonuclease III.

Authors:  T Roldán-Arjona; M V García-Ortiz; M Ruiz-Rubio; R R Ariza
Journal:  Plant Mol Biol       Date:  2000-09       Impact factor: 4.076

2.  Hairpin and parallel quartet structures for telomeric sequences.

Authors:  P Balagurumoorthy; S K Brahmachari; D Mohanty; M Bansal; V Sasisekharan
Journal:  Nucleic Acids Res       Date:  1992-08-11       Impact factor: 16.971

3.  The coherence of synthetic telomeres.

Authors:  O L Acevedo; L A Dickinson; T J Macke; C A Thomas
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

4.  Base-specific reactions useful for DNA sequencing: methylene blue--sensitized photooxidation of guanine and osmium tetraoxide modification of thymine.

Authors:  T Friedmann; D M Brown
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971

5.  Reactivity of cytosine and thymine in single-base-pair mismatches with hydroxylamine and osmium tetroxide and its application to the study of mutations.

Authors:  R G Cotton; N R Rodrigues; R D Campbell
Journal:  Proc Natl Acad Sci U S A       Date:  1988-06       Impact factor: 11.205

6.  Intermediate range effects in DNA. I: Low pH/stress induced conformational changes in the vicinity of an extruded d(AT)n.d(AT) in cruciform.

Authors:  J N Glover; D B Haniford; D E Pulleyblank
Journal:  Nucleic Acids Res       Date:  1988-06-24       Impact factor: 16.971

7.  Observations on nucleolar staining with osmium tetroxide.

Authors:  J C Stockert; O D Colman
Journal:  Experientia       Date:  1974-07-15

8.  Evaluation of phosphodiesterase I-based protocols for the detection of multiply damaged sites in DNA: the detection of abasic, oxidative and alkylative tandem damage in DNA oligonucleotides.

Authors:  K J Bowman; R L Pla; Y Guichard; P B Farmer; G D Jones
Journal:  Nucleic Acids Res       Date:  2001-10-15       Impact factor: 16.971

9.  Molecular cloning and functional analysis of a Schizosaccharomyces pombe homologue of Escherichia coli endonuclease III.

Authors:  T Roldán-Arjona; C Anselmino; T Lindahl
Journal:  Nucleic Acids Res       Date:  1996-09-01       Impact factor: 16.971

10.  Oxidation of pyrimidine nucleosides and nucleotides by osmium tetroxide.

Authors:  K Burton
Journal:  Biochem J       Date:  1967-08       Impact factor: 3.857
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