Literature DB >> 5938659

The isolation and composition of helical protein microfibrils from Hevea brasiliensis latex.

B G Audley.   

Abstract

1. The microfibrils contained within the lutoid particles of Hevea brasiliensis latex obtained from young tissue have been isolated by methods based on low-speed centrifugation, isoelectric precipitation and gel filtration. 2. The isolated microfibrils behave as a single protein having an isoelectric point of about 4 as determined by paper electrophoresis. 3. The only components so far detected in the microfibrils are protein and possibly carbohydrate; nucleic acid appears to be absent. 4. The amino acid composition of the microfibril protein shows no unusual features. 5. In latex from the more mature laticiferous tissues of H. brasiliensis, the lutoid particles appear to be devoid of microfibrils or their protein decomposition products.

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Year:  1966        PMID: 5938659      PMCID: PMC1264836          DOI: 10.1042/bj0980335

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  7 in total

1.  Zone electrophoresis in starch gels and its application to studies of serum proteins.

Authors:  O SMITHIES
Journal:  Adv Protein Chem       Date:  1959

2.  [Experiments on the quantitative determination of amino acids on ion exchange columns and automatic recording of the results].

Authors:  K HANNIG
Journal:  Clin Chim Acta       Date:  1959-01       Impact factor: 3.786

3.  The proteins of Hevea brasiliensis Latex. 4. Isolation and characterization of crystalline hevein.

Authors:  B L ARCHER
Journal:  Biochem J       Date:  1960-05       Impact factor: 3.857

4.  A negative staining method for high resolution electron microscopy of viruses.

Authors:  S BRENNER; R W HORNE
Journal:  Biochim Biophys Acta       Date:  1959-07

5.  Starch gel electrophoresis in a discontinous system of buffers.

Authors:  M D POULIK
Journal:  Nature       Date:  1957-12-28       Impact factor: 49.962

6.  The proteins of Hevea brasiliensis latex. I. Protein constituents of fresh latex serum.

Authors:  B L ARCHER; B C SEKHAR
Journal:  Biochem J       Date:  1955-11       Impact factor: 3.857

7.  The estimation of threonine and serine in proteins.

Authors:  M W Rees
Journal:  Biochem J       Date:  1946       Impact factor: 3.857

  7 in total
  1 in total

1.  Rubber elongation factor (REF), a major allergen component in Hevea brasiliensis latex has amyloid properties.

Authors:  Karine Berthelot; Sophie Lecomte; Yannick Estevez; Bénédicte Coulary-Salin; Ahmed Bentaleb; Christophe Cullin; Alain Deffieux; Frédéric Peruch
Journal:  PLoS One       Date:  2012-10-25       Impact factor: 3.240

  1 in total

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