Literature DB >> 593071

A simplified colorimetric micromethod for determination of serum cholesterol.

R R Bhandaru, S R Srinivasan, P S Pargaonkar, G S Berenson.   

Abstract

A simple and rapid micromethod is described for quantitation of cholesterol in 5-10 microliter of serum using uranyl acetate to deproteinize the serum and o-phthalaldehyde reagent to develop color. Independent analyses of serum samples by the present method and by two reference methods, Abell-Kendall and automated colorimetric method, indicated accuracy of the micromethod (r = 0.99). Use of capillary blood specimens makes this procedure applicable for screening infants and small children.

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Year:  1977        PMID： 593071     DOI： 10.1007/bf02533337

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  5 in total

1.  Colorimetry of serum cholesterol with use of ferric acetate/uranyl acetate and ferrous sulfate/sulfuric acid reagents.

Authors:  D H Jung; H G Biggs; W R Moorehead
Journal:  Clin Chem       Date:  1975-09       Impact factor: 8.327

2.  Determination of cholesterol using o-phthalaldehyde.

Authors:  L L Rudel; M D Morris
Journal:  J Lipid Res       Date:  1973-05       Impact factor: 5.922

3.  A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity.

Authors:  L L ABEL; B B LEVY; B B BRODIE; F E KENDALL
Journal:  J Biol Chem       Date:  1952-03       Impact factor: 5.157

4.  Uranyl acetate as an excellent fixative for lipoproteins after electrophoresis on agarose gel.

Authors:  A Lózsa
Journal:  Clin Chim Acta       Date:  1974-05-31       Impact factor: 3.786

5.  Study of a new cholesterol reagent.

Authors:  A Zlatkis; B Zak
Journal:  Anal Biochem       Date:  1969-04-11       Impact factor: 3.365
  5 in total
  7 in total

1.  Hypocholesterolemic activity of racemic dichlorophenoxypropionic acid or its enantiomers in rats.

Authors:  F Ohta; F Gaudemer; C Lutton
Journal:  Bull Environ Contam Toxicol       Date:  1987-12       Impact factor: 2.151

2.  Myelination of regenerating sciatic nerve of the rat: lipid components and synthesis of myelin lipids.

Authors:  M Alberghina; M Viola; F Moschella; A M Giuffrida
Journal:  Neurochem Res       Date:  1983-02       Impact factor: 3.996

3.  Effect of airborne fluoride on some hematological parameters of chick.

Authors:  K S Pillai; U H Mane
Journal:  Bull Environ Contam Toxicol       Date:  1985-10       Impact factor: 2.151

4.  The effect of dietary lipid on the lipoprotein status of the Mongolian gerbil.

Authors:  A B Leach; B J Holub
Journal:  Lipids       Date:  1984-01       Impact factor: 1.880

5.  A pivotal role of the human kidney in catabolism of HDL protein components apolipoprotein A-I and A-IV but not of A-II.

Authors:  Jonas Heilskov Graversen; Graciela Castro; Abdelmejid Kandoussi; Henning Nielsen; Erik Ilsø Christensen; Anthony Norden; Søren Kragh Moestrup
Journal:  Lipids       Date:  2008-03-19       Impact factor: 1.880

6.  Alteration and recovery of bleeding times, platelet aggregation and fatty acid composition of individual phospholipids in platelets of human subjects receiving a supplement of cod-liver oil.

Authors:  A A Ahmed; B J Holub
Journal:  Lipids       Date:  1984-08       Impact factor: 1.880

7.  Interaction between ovine growth hormone and triiodo-L-thyronine on metabolic reserves of rainbow trout,Salmo gairdneri.

Authors:  K J Farbridge; J F Leatherland
Journal:  Fish Physiol Biochem       Date:  1988-07       Impact factor: 2.794
  7 in total

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