Literature DB >> 5918715

Chloroplast DNA from tobacco leaves.

K K Tewari, S G Wildman.   

Abstract

DNA from tobacco leaf chloroplasts was isolated as a single component with a buoyant density in CsCl of 1.702 compared to 1.697 for nuclear DNA. 5-Methylcytosine is present in nuclear DNA but absent in chloroplast DNA. Chloroplast DNA, with a guanine-cytosine content of 43 percent, has a melting temperature of 86 degrees C and renatures completely on slow cooling, whereas nuclear DNA ( melting temperature, 84 degrees C; guanine-cytosine content, 40 percent) does not renature. About 9 percent of the total DNA in tobacco leaves is chloroplast DNA representing about 4.7 xX 10-(15) gram of DNA per chloroplast with a molecular weight of approximately 4 xX 10(7).

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Year:  1966        PMID： 5918715     DOI： 10.1126/science.153.3741.1269

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  20 in total

1.  Studies with deoxyribonucleic Acid from blue-green algae.

Authors:  S D Kung; M A Moscarello; J P Williams
Journal:  Plant Physiol       Date:  1972-03       Impact factor: 8.340

2.  Changes in Chloroplast DNA Levels during Development of Pea (Pisum sativum).

Authors:  G K Lamppa; A J Bendich
Journal:  Plant Physiol       Date:  1979-07       Impact factor: 8.340

3.  Chloroplast genes transferred to the nuclear plant genome have adjusted to nuclear base composition and codon usage.

Authors:  J L Oliver; A Marín; J M Martínez-Zapater
Journal:  Nucleic Acids Res       Date:  1990-01-11       Impact factor: 16.971

Review 4.  A comparison of the fine structure and nucleic acid biochemistry of chloroplasts and blue-green algae.

Authors:  B A Whitton; N G Carr; I W Craig
Journal:  Protoplasma       Date:  1971       Impact factor: 3.356

Review 5.  [Nucleic acids. Architecture and flexibility in the molecular picture].

Authors:  A K Kleinschmidt
Journal:  Naturwissenschaften       Date:  1967-08

6.  Studies on spinach chloroplast and nuclear DNA using large-scale tissue preparations.

Authors:  S A Bard; M P Gordon
Journal:  Plant Physiol       Date:  1969-03       Impact factor: 8.340

7.  DNA polymerase in isolated tobacco chloroplasts and nature of the polymerized product.

Authors:  K K Tewari; S G Wildman
Journal:  Proc Natl Acad Sci U S A       Date:  1967-08       Impact factor: 11.205

8.  Buoyant density studies of chloroplast and nuclear deoxyribonucleic acid from control and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare.

Authors:  P G Bartels; A Hyde
Journal:  Plant Physiol       Date:  1970-12       Impact factor: 8.340

9.  Flower Formation in Excised Tobacco Stem Segments: III. Deoxyribonucleic Acid Content in Stem Tissue of Vegetative and Flowering Tobacco Plants.

Authors:  W L Wardell
Journal:  Plant Physiol       Date:  1973-09       Impact factor: 8.340

10.  Identification of a mutation in chloroplast DNA correlated with formation of defective chloroplasts in a variegated mutant of Nicotiana tabacum.

Authors:  F Wong-Staal; S G Wildman
Journal:  Planta       Date:  1973-12       Impact factor: 4.116
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