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Literature DB >> 5862403

High-efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins.

Abstract

1. Inexpensive scintillation mixtures are described which enable the detection of as little as 40mumuc of (14)C in aqueous solution with an efficiency of counting of over 80%. 2. A rapid method for the counting of alkaline, acidic and neutral aqueous solutions of up to 1ml. volume is described. Ethanol or 2-ethoxyethanol is used as blending agent. 3. The scintillation counting of alkaline solutions is applied to the accurate determination of the specific activity of (14)C-labelled proteins from plant tissues. 4. Attention has been paid to the importance of a standardized washing procedure for the removal of all traces of radioactive material from glassware.

Entities: Chemical

Mesh：

Substances：

Year: 1965 PMID： 5862403 PMCID： PMC1207196 DOI： 10.1042/bj0960626

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

7 in total

1. Liquid scintillation-counting for [C-14]steroids.

Authors: S R STITCH
Journal: Biochem J Date: 1959-10 Impact factor: 3.857

2. [Comparative studies of quantitative determination of proteins in cerebrospinal fluid and low-protein solutions].

Authors: M EGGSTEIN; F H KREUTZ
Journal: Klin Wochenschr Date: 1955-10-01

3. Liquid-scintillation counting of C-labelled animal tissues at high efficiency.

Authors: W O Brown
Journal: Biochem J Date: 1961-03 Impact factor: 3.857

4. Binding sites as a limiting factor in amino acid incorporation.

Authors: C L ROBINSON; G D NOVELLI
Journal: Arch Biochem Biophys Date: 1962-03 Impact factor: 4.013

5. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

6. Uptake of radioactive alanine in vitro into the proteins of rat liver fractions.

Authors: P SIEKEVITZ
Journal: J Biol Chem Date: 1952-04 Impact factor: 5.157

7. The effects of x-irradiation on the incorporation of amino acids into proteins of nuclei from rat thymus gland.

Authors: J A Smit; L A Stocken
Journal: Biochem J Date: 1964-04 Impact factor: 3.857

7 in total

41 in total

1. The maximum activities of hexokinase, phosphorylase, phosphofructokinase, glycerol phosphate dehydrogenases, lactate dehydrogenase, octopine dehydrogenase, phosphoenolpyruvate carboxykinase, nucleoside diphosphatekinase, glutamate-oxaloacetate transaminase and arginine kinase in relation to carbohydrate utilization in muscles from marine invertebrates.

Authors: V A Zammit; E A Newsholme
Journal: Biochem J Date: 1976-12-15 Impact factor: 3.857

2. Pipemidic acid: absorption, distribution, and excretion.

Authors: M Shimizu; S Nakamura; Y Takase; N Kurobe
Journal: Antimicrob Agents Chemother Date: 1975-04 Impact factor: 5.191

3. Proteoglycan-degrading enzymes of rabbit fibroblasts and granulocytes.

Authors: Z Werb; J T Dingle; J J Reynolds; A J Barrett
Journal: Biochem J Date: 1978-09-01 Impact factor: 3.857

4. Interaction of human cathepsin D with the inhibitor pepstatin.

Authors: C G Knight; A J Barrett
Journal: Biochem J Date: 1976-04-01 Impact factor: 3.857

5. The cytidine mechanism and methylation pathway in the formation of N-methylated ethanolamine phosphoglycerides in Neurospora crassa.

Authors: Z Matysiak; A Radomińska-Pyrek; T Chojnacki
Journal: Mol Cell Biochem Date: 1974-04-15 Impact factor: 3.396