Conditions for competence in the Bacillus licheniformis transformation system.

Some variables in the Bacillus licheniformis transformation system, such as inoculum size, initial cell density in transformation medium, and time of optimal competence, have been explored, and a methodology has been developed to obtain greater reproducibility and maximal competence for transformation. The transformation system in B. licheniformis has been shown to be similar to that of B. subtilis in a number of respects, including "dormancy" of transformed cells and the concentration of transforming deoxyribonucleic acid required for saturation.