Literature DB >> 14197892

TRANSFORMATION TO PROTOTROPHY AND POLYGLUTAMIC ACID SYNTHESIS IN BACILLUS LICHENIFORMIS.

C G LEONARD, D K MATTHEIS, M J MATTHEIS, R D HOUSEWRIGHT.   

Abstract

Leonard, C. Gomez (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), D. K. Mattheis, M. J. Mattheis, and R. D. Housewright. Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis. J. Bacteriol. 88:220-225. 1964.-Growth conditions necessary for transformation to prototrophy of 14 auxotrophs of Bacillus licheniformis were studied. The unexpected finding of different growth requirements by each auxotroph for the development of transformable cells is discussed. Under optimal growth conditions for a serine-deficient mutant transformation frequencies of 0.1% were obtained. A medium for transformation of competent cells in liquid suspensions is described. In addition, this report presents evidence for the transformation of three nonencapsulated mutants of B. licheniformis for the ability to synthesize polyglutamic acid (capsular material).

Entities:  

Keywords:  BACILLUS; EXPERIMENTAL LAB STUDY; GENETICS; GLUTAMATES; METABOLISM; MUTATION

Mesh:

Substances:

Year:  1964        PMID: 14197892      PMCID: PMC277280          DOI: 10.1128/jb.88.1.220-225.1964

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  13 in total

1.  POLYGLUTAMIC ACID SYNTHESIS BY CELL-FREE EXTRACTS OF BACILLUS LICHENIFORMIS.

Authors:  C G LEONARD; R D HOUSEWRIGHT
Journal:  Biochim Biophys Acta       Date:  1963-07-09

2.  Transformability of Haemophilus influenzae.

Authors:  J H STUY
Journal:  J Gen Microbiol       Date:  1962-11

3.  Effects of some metallic ions on glutamyl polypeptide synthesis by Bacillus subtilis.

Authors:  C G LEONARD; R D HOUSEWRIGHT; C B THORNE
Journal:  J Bacteriol       Date:  1958-11       Impact factor: 3.490

4.  Isolation of D- and L-glutamyl polypeptides from culture filtrates of Bacillus subtilis.

Authors:  C B THORNE; C G LEONARD
Journal:  J Biol Chem       Date:  1958-11       Impact factor: 5.157

5.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

6.  CONCENTRATION AND ISOLATION OF AUXOTROPHIC MUTANTS OF SPOREFORMING BACTERIA.

Authors:  V Iyer
Journal:  J Bacteriol       Date:  1960-02       Impact factor: 3.490

7.  REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors:  C Anagnostopoulos; J Spizizen
Journal:  J Bacteriol       Date:  1961-05       Impact factor: 3.490

8.  Transduction in Bacillus subtilis.

Authors:  C B THORNE
Journal:  J Bacteriol       Date:  1962-01       Impact factor: 3.490

9.  INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM.

Authors:  F E YOUNG; J SPIZIZEN
Journal:  J Bacteriol       Date:  1963-09       Impact factor: 3.490

10.  Studies on transformations of Hemophilus influenzae. I. Competence.

Authors:  S H GOODGAL; R M HERRIOTT
Journal:  J Gen Physiol       Date:  1961-07       Impact factor: 4.086

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  12 in total

1.  Instability of protease production in a rel+/rel- -pair of Bacillus licheniformis and associated morphological and physiological characteristics.

Authors:  B A Bulthuis; J Frankena; G M Koningstein; H W van Verseveld; A H Stouthamer
Journal:  Antonie Van Leeuwenhoek       Date:  1988       Impact factor: 2.271

2.  Identification of competence-repressing factors during log-phase growth of Haemophilus influenzae.

Authors:  D H Miller; P C Huang
Journal:  J Bacteriol       Date:  1972-02       Impact factor: 3.490

3.  Sequential replication of the chromosome of Bacillus licheniformis.

Authors:  F J Tyeryar; W D Lawton; A M MacQuillan
Journal:  J Bacteriol       Date:  1968-06       Impact factor: 3.490

4.  Factors affecting transformation of Micrococcus lysodeikticus.

Authors:  W E Kloos
Journal:  J Bacteriol       Date:  1969-06       Impact factor: 3.490

5.  Facilitation of direct conditional knockout of essential genes in Bacillus licheniformis DSM13 by comparative genetic analysis and manipulation of genetic competence.

Authors:  Kerstin Hoffmann; Antje Wollherr; Michael Larsen; Michael Rachinger; Heiko Liesegang; Armin Ehrenreich; Friedhelm Meinhardt
Journal:  Appl Environ Microbiol       Date:  2010-06-11       Impact factor: 4.792

6.  Factors affecting transformation of Bacillus licheniformis.

Authors:  C B Thorne; H B Stull
Journal:  J Bacteriol       Date:  1966-03       Impact factor: 3.490

7.  Factors affecting transfection in Bacillus stearothermophilus.

Authors:  U N Streips; N E Welker
Journal:  J Bacteriol       Date:  1971-06       Impact factor: 3.490

8.  Conditions affecting transformation of a group H streptococcus.

Authors:  H J Schlissel; C P Sword
Journal:  J Bacteriol       Date:  1966-11       Impact factor: 3.490

9.  Genetic mapping of genes concerned with glutamyl polypeptide production by Bacillus licheniformis and a study of their relationship to the development of competence for transformation.

Authors:  R W McCuen; C B Thorne
Journal:  J Bacteriol       Date:  1971-09       Impact factor: 3.490

10.  Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus.

Authors:  G R Germaine; D L Anderson
Journal:  J Bacteriol       Date:  1966-09       Impact factor: 3.490

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