Literature DB >> 5775690

Enzymic degradation of heparin. A sulphamidase and a sulphoesterase from Flavobacterium heparinum.

C P Dietrich.   

Abstract

A sulphamidase and a sulphoesterase were isolated from adapted cells of Flavobacterium heparinum. These enzymes were partially purified from the ;heparinases' present in the bacterial extracts and characterized. The sulphamidase has a high specificity for glucosamine N-sulphate and glucosamine 2,6-disulphate. The activity decreases sharply with increasing molecular weight of the substrates tested. The sulphamidase and the sulphoesterase activities were distinguished from each other by their different sensitivities to concentration of phosphate ion and to temperature. The importance of these enzymes in the study of the structure of heparin is discussed.

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Year:  1969        PMID： 5775690      PMCID： PMC1187498          DOI： 10.1042/bj1110091

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  6 in total

1.  The enzymic degradation of heparitin sulfate.

Authors:  A LINKER; P SAMPSON
Journal:  Biochim Biophys Acta       Date:  1960-09-23

2.  The degradation of heparin by bacterial enzymes. III. A comparison of the degradation of heparin, hyaluronic acid, and chondroitin sulfate.

Authors:  E D KORN
Journal:  J Biol Chem       Date:  1957-06       Impact factor: 5.157

3.  The degradation of heparin by bacterial enzymes. II. Acetone powder extracts.

Authors:  E D KORN; A N PAYZA
Journal:  J Biol Chem       Date:  1956-12       Impact factor: 5.157

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  The enzymatic degradation of heparin and heparitin sulfate. I. The fractionation of a crude heparinase from flavobacteria.

Authors:  A Linker; P Hovingh
Journal:  J Biol Chem       Date:  1965-10       Impact factor: 5.157

6.  Novel heparin degradation products. Isolation and characterization of novel disaccharides and oligosaccharides produced from heparin by bacterial degradation.

Authors:  C P Dietrich
Journal:  Biochem J       Date:  1968-07       Impact factor: 3.857
  6 in total
  7 in total

1.  A comparative study between a chondroitinase B and a chondroitinase AC from Flavobacterium heparinum: Isolation of a chondroitinase AC-susceptible dodecasaccharide from chondroitin sulphate B.

Authors:  Y M Michelacci; C P Dietrich
Journal:  Biochem J       Date:  1975-10       Impact factor: 3.857

2.  Large scale preparation and characterization of mucopolysaccharase contamination free heparinase.

Authors:  V C Yang; H Bernstein; C L Cooney; R Langer
Journal:  Appl Biochem Biotechnol       Date:  1987 Sep-Dec       Impact factor: 2.926

3.  Abnormal arylsulphatase activities of fibroblasts cultured from patients with mucopolysaccharidosis and cystinosis.

Authors:  K Furusho; M Vetrella; E Latta
Journal:  Z Kinderheilkd       Date:  1971

4.  Studies on the particle-bound carbohydrate sulphoamide sulphohydrolase and carbohydrate sulphate sulphohydrolase of Flavobacterium heparinum.

Authors:  B A Law; A G Lloyd; G Embery; G B Wisdom
Journal:  Biochem J       Date:  1969-11       Impact factor: 3.857

Review 5.  Sulfate ester formation and hydrolysis: a potentially important yet often ignored aspect of the sulfur cycle of aerobic soils.

Authors:  J W Fitzgerald
Journal:  Bacteriol Rev       Date:  1976-09

6.  Structure of heparin-derived tetrasaccharides.

Authors:  Z M Merchant; Y S Kim; K G Rice; R J Linhardt
Journal:  Biochem J       Date:  1985-07-15       Impact factor: 3.857

7.  Sulfur regulation of heparinase and sulfatases in Flavobacterium heparinum.

Authors:  E C Cerbelaud; L J Conway; P M Galliher; R S Langer; C L Cooney
Journal:  Appl Environ Microbiol       Date:  1986-03       Impact factor: 4.792
  7 in total

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