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Literature DB >> 574514

High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements.

Abstract

Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells.

Entities: Chemical

Mesh：

Substances：
Actins

Year: 1979 PMID： 574514 PMCID： PMC2110435 DOI： 10.1083/jcb.83.1.249

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

19 in total

1. Cytoskeletal elements of chick embryo fibroblasts revealed by detergent extraction.

Authors: S Brown; W Levinson; J A Spudich
Journal: J Supramol Struct Date: 1976

2. Three-dimensional electron microscopical visualization of the cytoskeleton of animal cells: immunoferritin identification of actin- and tubulin-containing structures.

Authors: R E Webster; D Henderson; M Osborn; K Weber
Journal: Proc Natl Acad Sci U S A Date: 1978-11 Impact factor: 11.205

3. Selective binding of antibody against gizzard 10-nm filaments to different cell types in myogenic cultures.

Authors: S A Fellini; G S Bennett; H Holtzer
Journal: Am J Anat Date: 1978-11

4. Visualization of the same PtK2 cytoskeletons by both immunofluorescence and low power electron microscopy.

Authors: R E Webster; M Osborn; K Weber
Journal: Exp Cell Res Date: 1978-11 Impact factor: 3.905

5. The distribution of desmin (100 A) filaments in primary cultures of embryonic chick cardiac cells.

Authors: E Lazarides
Journal: Exp Cell Res Date: 1978-03-15 Impact factor: 3.905

6. The sub-membrane reticulum of the human erythrocyte: a scanning electron microscope study.

Authors: J F Hainfeld; T L Steck
Journal: J Supramol Struct Date: 1977

7. The existence of an insoluble Z disc scaffold in chicken skeletal muscle.

Authors: B L Granger; E Lazarides
Journal: Cell Date: 1978-12 Impact factor: 41.582

8. Studies on the function and composition of the 10-NM(100-A) filaments of vertebrate smooth muscle.

Authors: J V Small; A Sobieszek
Journal: J Cell Sci Date: 1977-02 Impact factor: 5.285

9. Characterization of the nuclear envelope, pore complexes, and dense lamina of mouse liver nuclei by high resolution scanning electron microscopy.

Authors: R H Kirschner; M Rusli; T E Martin
Journal: J Cell Biol Date: 1977-01 Impact factor: 10.539

10. Biochemical and immunological analysis of rapidly purified 10-nm filaments from baby hamster kidney (BHK-21) cells.

Authors: J M Starger; W E Brown; A E Goldman; R D Goldman
Journal: J Cell Biol Date: 1978-07 Impact factor: 10.539

18 in total

1. Immunolocalization in three dimensions: immunogold staining of cytoskeletal and nuclear matrix proteins in resinless electron microscopy sections.

Authors: J A Nickerson; G Krockmalnic; D C He; S Penman
Journal: Proc Natl Acad Sci U S A Date: 1990-03 Impact factor: 11.205

2. Globular and fibrous structure in barley chromosomes revealed by high-resolution scanning electron microscopy.

Authors: M Iwano; K Fukui; S Takaichi; A Isogai
Journal: Chromosome Res Date: 1997-08 Impact factor: 5.239

3. The organization of the mammalian kinetochore: a scanning electron microscope study.

Authors: J B Rattner
Journal: Chromosoma Date: 1987 Impact factor: 4.316

4. Chromosomal localization of genes by scanning electron microscopy using in situ hybridization with biotinylated probes: Y chromosome repetitive sequences.

Authors: D J Ferguson; J Burns; D Harrison; J A Jonasson; J O McGee
Journal: Histochem J Date: 1986-05

9. Scanning electron microscopy of the centromeric region of L-cell chromosomes after treatment with Hoechst 33258 combined with 5-bromodeoxyuridine.

Authors: S Takayama; H Hiramatsu
Journal: Chromosoma Date: 1993-03 Impact factor: 4.316

10. High-order structure of metaphase chromosomes: evidence for a multiple coiling model.

Authors: T Taniguchi; S Takayama
Journal: Chromosoma Date: 1986 Impact factor: 4.316