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Literature DB >> 573890

Cloning of soybean leghemoglobin structural gene sequences synthesized in vitro.

E Truelsen, K Gausing, B Jochimsen, P Jørgensen, K A Marcker.

Abstract

Double-stranded soybean leghemoglobin DNA was synthesized from leghemoglobin mRNA isolated from soybean nodules. The dsDNA was inserted into the Bam H1 site of plasmid pBR322 using the poly-dAT-joiner method. A cloned DNA fragment of one recombinant plasmid was isolated and characterized by restriction endonuclease digestion. The restriction cleavage map and the DNA sequence of a selected part of the inserted DNA are in complete accordance with the amino-acid sequence of soybean leghemoglobin.

Entities: Gene Species

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Year: 1979 PMID： 573890 PMCID： PMC327917 DOI： 10.1093/nar/6.9.3061

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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References

22 in total

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Authors: M P Wickens; G N Buell; R T Schimke
Journal: J Biol Chem Date: 1978-04-10 Impact factor: 5.157

Cloning of soybean leghemoglobin structural gene sequences synthesized in vitro.

1. Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA.

2. DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli.

3. Comparison of cloned rabbit and mouse beta-globin genes showing strong evolutionary divergence of two homologous pairs of introns.

4. Construction and identification by positive hybridization-translation of a bacterial plasmid containing a rat growth hormone structural gene sequence.

5. Immunoglobulin light-chain structural gene sequences cloned in a bacterial plasmid.

6. A method for the recovery of DNA from agarose gels.

7. The syntheiss of high yields of full-length reverse transcripts of globin mRNA.

8. Specific excision of the inserted DNA segment from hybrid plasmids constructed by the poly(dA). poly (dT) method.

9. Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation.

10. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.