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Literature DB >> 5688926

The distribution of glutamate decarboxylase and aspartate transaminase in subcellular fractions of rat and guinea-pig brain.

Abstract

1. The subcellular distributions of glutamate decarboxylase and aspartate transaminase were studied in rat and guinea-pig brain. 2. Glutamate decarboxylase is localized in the synaptosome fraction. The mean density of the particles containing the enzyme is slightly greater than those derived from cholinergic neurones, though overlap is substantial. 3. The enzyme is readily released from synaptosomes by hypo-osmotic treatment, but in the presence of Ca(2+), Na(+) and K(+) it sediments with particulate material. 4. The release and binding of the enzyme to membrane fractions by Ca(2+) were investigated. 5. Aspartate transaminase is present in brain as two isoenzymes with different kinetic properties. One isoenzyme is associated with the cytoplasm and the other with mitochondria.

Entities: Chemical Gene Species

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Year: 1968 PMID： 5688926 PMCID： PMC1198516 DOI： 10.1042/bj1060401

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

29 in total

1. CEREBRAL LYSOSOMES--I. A COMPARATIVE STUDY OF LYSOSOMAL N-ACETYL-BETA-D-GLUCOSAMINIDASE AND MITOCHONDRIAL ASPARTIC TRANSAMINASE OF RAT CEREBRAL CORTEX.

Authors: O Z SELLINGER; D L RUCKER; F DE BALBIAN VERSTER
Journal: J Neurochem Date: 1964-04 Impact factor: 5.372

2. ON THE INTRACELLULAR LOCALIZATION OF GLUTAMATE DECARBOXYLASE AND GAMMA-AMINOBUTYRIC ACID IN MAMMALIAN BRAIN.

Authors: N F SHATUNOVA; I A SYTINSKY
Journal: J Neurochem Date: 1964-10 Impact factor: 5.372

3. LACTATE DEHYDROGENASE AS A CYTOPLASMIC MARKER IN BRAIN.

Authors: M K JOHNSON; V P WHITTAKER
Journal: Biochem J Date: 1963-09 Impact factor: 3.857

4. Multiple forms of glutamic-oxalacetic transaminase in tissues.

Authors: L E DECKER; E M RAU
Journal: Proc Soc Exp Biol Med Date: 1963-01

5. The subcellular localization of 5-hydroxytryptamine in guinea pig brain.

Authors: I A MICHAELSON; V P WHITTAKER
Journal: Biochem Pharmacol Date: 1963-02 Impact factor: 5.858

6. Studies of subcellular distribution of gamma-aminobutyric acid and glutamic decarboxylase in mouse brain.

Authors: H WEINSTEIN; E ROBERTS; T KAKEFUDA
Journal: Biochem Pharmacol Date: 1963-05 Impact factor: 5.858

7. Intracellular localization of glutamate decarboxylase, gamma-aminobutyrate transaminase and some other enzymes in brain tissue.

Authors: G M van Kempen; C J van den Berg; H J van der Helm; H Veldstra
Journal: J Neurochem Date: 1965-07 Impact factor: 5.372

8. The morphology of fractions of rat forebrain synaptosomes separated on continuous sucrose density gradients.

Authors: V P Whittaker
Journal: Biochem J Date: 1968-01 Impact factor: 3.857

9. The osmotically sensitive potassium and sodium compartments of synaptosomes.

Authors: R M Marchbanks
Journal: Biochem J Date: 1967-07 Impact factor: 3.857

10. A radiochemical method for the estimation of choline acetyltransferase.

Authors: F Fonnum
Journal: Biochem J Date: 1966-08 Impact factor: 3.857

32 in total

1. Is there a high molecular weight glutamic acid decarboxylase?

Authors: M Pérez-de la Mora; A B Rizo-Silva; J Méndez-Franco
Journal: Neurochem Res Date: 1992-04 Impact factor: 3.996

Review 2. The structural and functional heterogeneity of glutamic acid decarboxylase: a review.

Authors: M G Erlander; A J Tobin
Journal: Neurochem Res Date: 1991-03 Impact factor: 3.996

3. Changes in some enzymic activities of separated neuronal and glial cell-enriched fractions from rat brains during development.

Authors: Y Nagata; T Nanba; M Ando
Journal: Neurochem Res Date: 1976-06 Impact factor: 3.996

4. Electrical stimulation-evoked release of endogenous aspartate from rat medulla oblongata slices. Effects of inhibitors of aspartate aminotransferase and GABA transaminase.

Authors: T Kubo; M Kihara; Y Misu
Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 1990-03 Impact factor: 3.000

5. Comparative studies on glutamate metabolism in synpatic and non-synaptic rat brain mitochondria.

Authors: S C Dennis; J C Lai; J B Clark
Journal: Biochem J Date: 1977-06-15 Impact factor: 3.857

6. Distribution and characterization of the GABA receptors in the CNS of ataxic mutant mouse.

Authors: T Yamaguchi; K Hayashi; H Murakami; S Maruyama; M Yamaguchi
Journal: Neurochem Res Date: 1984-04 Impact factor: 3.996

7. Rat cerebral-cortex adenosine deaminase activity and its subcellular distribution.

Authors: I Pull; H McIlwain
Journal: Biochem J Date: 1974-10 Impact factor: 3.857

8. A membrane form of brain L-glutamate decarboxylase: identification, isolation, and its relation to insulin-dependent mellitus.

Authors: B Nathan; J Bao; C C Hsu; P Aguilar; R Wu; M Yarom; C Y Kuo; J Y Wu
Journal: Proc Natl Acad Sci U S A Date: 1994-01-04 Impact factor: 11.205

9. gamma-Aminobutyric acid system in isolated dorsal and ventral horn neurons from bovine spinal cord.

Authors: M Wakabayashi; H Higa; E Kushiya; K Araki; Y Takahashi
Journal: Neurochem Res Date: 1981-06 Impact factor: 3.996

10. Effects of 4-methylimidazole on cerebral glutamate decarboxylase activity and specific GABA receptor binding in mice.

Authors: Tore Sivertsen; Ann-Kristin Nygaard; Gro Mathisen; Frode Fonnum
Journal: Toxicol Mech Methods Date: 2009-03 Impact factor: 2.987