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Literature DB >> 5664107

A precise method for replicating suspension cultures of mammalian cells.

Abstract

A simple, readily assembled shaker-culture system for the cultivation of mammalian cells is described. No specialized glassware and equipment were used in this system, which consists of an Erlenmeyer flask fitted with a breather-sampling assembly. This unit was employed to quantitate the effects of several variables, including medium ingredients, serial transfers, and freezing and storage on two variants of the L-cell line. This system is reproducible and precise and allows for growth of cells in suspension for extended periods of time. Large numbers of cells can be mass-produced. Many replicates can be run simultaneously to yield data for statistical analysis.

Entities: Species

Mesh：

Substances：
Culture Media

Year: 1968 PMID： 5664107 PMCID： PMC547539 DOI： 10.1128/am.16.6.841-844.1968

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

10 in total

1. ENVIRONMENTAL INFLUENCES ON GROWTH OF L AND ERK MAMMALIAN CELLS IN SHAKE-FLASK CULTURES.

Authors: S J PIRT; E J THACKERAY
Journal: Exp Cell Res Date: 1964-02 Impact factor: 3.905

2. CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.

Authors: R G HAM
Journal: Proc Natl Acad Sci U S A Date: 1965-02 Impact factor: 11.205

3. Growth of cells in agitated fluid medium.

Authors: O VON H OWENS; M K GEY; G O GEY
Journal: Ann N Y Acad Sci Date: 1954-11-17 Impact factor: 5.691

4. Certain factors limiting the size of the tissue culture and the development of massive cultures.

Authors: W R EARLE; J C BRYANT; E L SCHILLING
Journal: Ann N Y Acad Sci Date: 1954-11-17 Impact factor: 5.691

5. Proliferation of monkey kidney cells in rotating cultures.

Authors: A F GRAHAM; L SIMINOVITCH
Journal: Proc Soc Exp Biol Med Date: 1955-06

6. Growth of cell suspensions in tissue culture.

Authors: W R EARLE; J C BRYANT; E L SCHILLING; V J EVANS
Journal: Ann N Y Acad Sci Date: 1956-03-14 Impact factor: 5.691

7. Propagation of strain L (Earle) cells in agitated fluid suspension cultures.

Authors: R J KUCHLER; D J MERCHANT
Journal: Proc Soc Exp Biol Med Date: 1956 Aug-Sep

8. The growth of pure strain L cells in fluid-suspension cultures.

Authors: W R EARLE; E L SCHILLING; J C BRYANT; V J EVANS
Journal: J Natl Cancer Inst Date: 1954-04 Impact factor: 13.506

9. Influence of tube rotation velocity on proliferation of strain L cells in surface substrate roller-tube cultures.

Authors: W R EARLE; E L SCHILLING; J C BRYANT
Journal: J Natl Cancer Inst Date: 1954-02 Impact factor: 13.506

10. The growth of animal cells in bacteriological media.

Authors: C P Kenny; H Lees
Journal: Can J Biochem Date: 1965-07

10 in total

1. Large-scale production and concentration of infectious Epstein-Barr virus.

Authors: F Klein; J F Rosensteel; R M Hummer; E A Hillman; C W Riggs; L J Charmella
Journal: Appl Environ Microbiol Date: 1978-01 Impact factor: 4.792

2. Growth of Chlamydia psittaci strain meningopneumonitis in mouse L cells cultivated in a defined medium in spinner cultures.

Authors: S J Morrison; H M Jenkin
Journal: In Vitro Date: 1972 Sep-Oct

3. Effect of osmotic pressure, Na+-K+ ratio and medium concentration on the enzyme activity and growth of L cells in suspension culture.

Authors: D C Fish; J P Dobbs; J M Elliott
Journal: In Vitro Date: 1973 Sep-Oct

7. Changes in enzyme specific activity and isoenzyme distribution patterns of L cells as a function of growth conditions.

Authors: D C Fish; J P Dobbs
Journal: In Vitro Date: 1971 May-Jun

8. Interacting factors that influence long-term storage of live Pasteurella tularensis vaccine and Rift Valley fever virus.

Authors: F Klein; J S Walker; B G Mahlandt; R C Carter; M D Orlando; F J Weirether; R E Lincoln
Journal: Appl Microbiol Date: 1969-03

9. Concentration of Rift Valley fever and Chikungunya viruses by precipitation.

Authors: F Klein; B G Mahlandt; R R Cockey; R E Lincoln
Journal: Appl Microbiol Date: 1970-09

10. Analysis and effect of conventional flasks in shaking culture of Escherichia coli.

Authors: Masato Takahashi; Hideki Aoyagi
Journal: AMB Express Date: 2020-04-19 Impact factor: 3.298

10 in total

A precise method for replicating suspension cultures of mammalian cells.

1. ENVIRONMENTAL INFLUENCES ON GROWTH OF L AND ERK MAMMALIAN CELLS IN SHAKE-FLASK CULTURES.

2. CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.

3. Growth of cells in agitated fluid medium.

4. Certain factors limiting the size of the tissue culture and the development of massive cultures.

5. Proliferation of monkey kidney cells in rotating cultures.

6. Growth of cell suspensions in tissue culture.

7. Propagation of strain L (Earle) cells in agitated fluid suspension cultures.

8. The growth of pure strain L cells in fluid-suspension cultures.

9. Influence of tube rotation velocity on proliferation of strain L cells in surface substrate roller-tube cultures.

10. The growth of animal cells in bacteriological media.

1. Large-scale production and concentration of infectious Epstein-Barr virus.

2. Growth of Chlamydia psittaci strain meningopneumonitis in mouse L cells cultivated in a defined medium in spinner cultures.

3. Effect of osmotic pressure, Na+-K+ ratio and medium concentration on the enzyme activity and growth of L cells in suspension culture.

4. Evaluation of methods for reestablishment of L-cell suspension cultures directly from liquid nitrogen stored stocks.

5. Growth of pathogenic virus in a large-scale tissue culture system.

6. Evaluation of factors related to growth of Rift Valley fever virus in suspended cell cultures.

7. Changes in enzyme specific activity and isoenzyme distribution patterns of L cells as a function of growth conditions.

8. Interacting factors that influence long-term storage of live Pasteurella tularensis vaccine and Rift Valley fever virus.

9. Concentration of Rift Valley fever and Chikungunya viruses by precipitation.

10. Analysis and effect of conventional flasks in shaking culture of Escherichia coli.