Literature DB >> 5494763

Concentration of Rift Valley fever and Chikungunya viruses by precipitation.

F Klein, B G Mahlandt, R R Cockey, R E Lincoln.   

Abstract

Simple and efficient methods for concentrating Rift Valley fever (RVF) virus and chikungunya (CHIK) virus are described. Ammonium sulfate, potassium sulfate, or alcohol was used as a precipitating agent and the precipitate was resuspended to volumes suitable for further processing and purification. The methods permitted concentration of live RVF virus and CHIK virus about 100-fold with negligible losses of virus. RVF virus retained a high level of infectivity with potassium aluminum sulfate and alcohol, but CHIK virus retained a higher infectivity level with ammonium sulfate than with potassium aluminum sulfate. The data indicate that serum plays an important role in the concentration of both viruses, at least when the sulfate methods are used.

Entities:  

Mesh：

Substances：

Year:  1970        PMID： 5494763      PMCID： PMC376937          DOI： 10.1128/am.20.3.346-350.1970

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  14 in total

1.  Purification of poliovirus labeled with radiophosphorus.

Authors:  J TAYLOR; A F GRAHAM
Journal:  Virology       Date:  1958-10       Impact factor: 3.616

2.  Simple method for preparation of purified radioactive poliovirus particles: electron micrograph.

Authors:  B H HOYER; E T BOLTON; D B RITTER; E RIBI
Journal:  Virology       Date:  1959-04       Impact factor: 3.616

3.  Column purification of influenza virus.

Authors:  L H FROMMHAGEN; C A KNIGHT
Journal:  Virology       Date:  1959-06       Impact factor: 3.616

4.  The purification and concentration of viruses and virus soluble antigens on calcium phosphate.

Authors:  J TAVERNE; J H MARSHALL; F FULTON
Journal:  J Gen Microbiol       Date:  1958-12

5.  The purification of poliomyelitis virus as studied by complement fixation.

Authors:  M M MAYER; H J RAPP; B ROIZMAN; S W KLEIN; K M COWAN; D LUKENS
Journal:  J Immunol       Date:  1957-06       Impact factor: 5.422

6.  Differentiation and purification of influenza viruses by adsorption on aluminum phosphate.

Authors:  H K MILLER; R W SCHLESINGER
Journal:  J Immunol       Date:  1955-08       Impact factor: 5.422

7.  Preparation of antiserum to enteroviruses in large animals.

Authors:  B Hampil; J L Melnick; C Wallis; R W Brown; E T Braye; R R Adams
Journal:  J Immunol       Date:  1965-11       Impact factor: 5.422

8.  A precise method for replicating suspension cultures of mammalian cells.

Authors:  F J Weirether; J S Walker; R E Lincoln
Journal:  Appl Microbiol       Date:  1968-06

9.  Evaluation of factors related to growth of Rift Valley fever virus in suspended cell cultures.

Authors:  J S Walker; R C Carter; F Klein; S E Snowden; R E Lincoln
Journal:  Appl Microbiol       Date:  1969-05

10.  Biological characteristics of plaque variants of Rift Valley fever virus.

Authors:  J J Boyle
Journal:  Am J Vet Res       Date:  1967-07       Impact factor: 1.156
View more
  3 in total

1.  Ultrafiltration as a method for concentrating Rift Valley fever virus grown in tissue culture.

Authors:  F Klein; B G Mahlandt; H B Bonner; R E Lincoln
Journal:  Appl Microbiol       Date:  1971-04

2.  Growth of pathogenic virus in a large-scale tissue culture system.

Authors:  F Klein; W I Jones; B G Mahlandt; R E Lincoln
Journal:  Appl Microbiol       Date:  1971-02

3.  A virus precipitation method for concentration & detection of avian influenza viruses from environmental water resources & its possible application in outbreak investigations.

Authors:  Shailesh D Pawar; Sachin S Keng; Deeksha S Tare; Anil L Thormothe; Gajanan N Sapkal; B Anukumar; Kavita S Lole; Jayati Mullick; Devendra T Mourya
Journal:  Indian J Med Res       Date:  2019-12       Impact factor: 2.375
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.