Literature DB >> 566121

Detection of a tetrahedral intermediate in the trypsin-catalysed hydrolysis of specific ring-activated anilides.

D D Petkov.   

Abstract

The substituent dependence for kcat/Km of trypsin anilide hydrolysis is consistent with a rate-limiting general acid-base catalysed breakdown of a tetrahedral intermediate. The formation and disappearance of this intermediate during the hydrolysis of alpha-N-acetyl-L-lysin p-nitroanilide is observed in stopped-flow experiments.

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Year:  1978        PMID： 566121     DOI： 10.1016/0005-2744(78)90057-8

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  4 in total

1.  Detection and accumulation of tetrahedral intermediates in elastase catalysis.

Authors:  A L Fink; P Meehan
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

Review 2.  Current problems in mechanistic studies of serine and cysteine proteinases.

Authors:  L Polgár; P Halász
Journal:  Biochem J       Date:  1982-10-01       Impact factor: 3.857

3.  Do cleavages of amides by serine proteases occur through a stepwise pathway involving tetrahedral intermediates?

Authors:  M Komiyama; M L Bender
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

4.  Chemical synthesis and papain-catalysed hydrolysis of N-alpha-benzyloxycarbonyl-L-lysine p-nitroanilide.

Authors:  N E Mackenzie; J P Malthouse; A I Scott
Journal:  Biochem J       Date:  1985-03-01       Impact factor: 3.857
  4 in total

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