Literature DB >> 564744

Gene mapping by fluorescent in situ hybridization.

S W Cheung, P V Tishler, L Atkins, S K Sengupta, E J Modest, B G Forget.   

Abstract

We describe a new method for the mapping of mammalian genes, utilizing in situ hybridization of mRNA to DNA of chromosomes. It involves the hydrogen bonding of the polyadenylic acid at the 3' end of hybridized mRNA to the polyuridylic acid tail of a highly fluorescent latex microsphere. The resultant double hybrid can be visualized by fluorescence microscopy. The chromosomal localization of human alpha + beta globin genes has been explored by this method. Our data point ot the long arms of chromosomes 4 and 5 as the loci for the human globin genes.

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Year:  1977        PMID： 564744     DOI： 10.1016/0309-1651(77)90050-9

Source DB:  PubMed          Journal:  Cell Biol Int Rep        ISSN： 0309-1651


  9 in total

1.  CHRONIS: an animal chromosome image database.

Authors:  Shin-Ichi Toyabe; Kouhei Akazawa; Daisuke Fukushi; Kiichi Fukui; Tatsuo Ushiki
Journal:  Chromosome Res       Date:  2005-09-21       Impact factor: 5.239

2.  Chromosomal localization of human beta globin gene on human chromosome 11 in somatic cell hybrids.

Authors:  A Deisseroth; A Nienhuis; J Lawrence; R Giles; P Turner; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

3.  Assignment of human beta-, gamma-, and delta-globin genes to the short arm of chromosome 11 by chromosome sorting and DNA restriction enzyme analysis.

Authors:  R V Lebo; A V Carrano; K Burkhart-Schultz; A M Dozy; L C Yu; Y W Kan
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

4.  DNA restriction endonuclease analysis for localization of human beta- and delta-globin genes on chromosome 11.

Authors:  A F Scott; J A Phillips; B R Migeon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Immunological method for mapping genes on Drosophila polytene chromosomes.

Authors:  P R Langer-Safer; M Levine; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

6.  Localization of the beta-globin gene by chromosomal in situ hybridization.

Authors:  C C Morton; I R Kirsch; R Taub; S H Orkin; J A Brown
Journal:  Am J Hum Genet       Date:  1984-05       Impact factor: 11.025

7.  Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analog.

Authors:  R H Singer; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

Review 8.  The dynamic architectural and epigenetic nuclear landscape: developing the genomic almanac of biology and disease.

Authors:  Phillip W L Tai; Sayyed K Zaidi; Hai Wu; Rodrigo A Grandy; Martin Montecino; André J van Wijnen; Jane B Lian; Gary S Stein; Janet L Stein
Journal:  J Cell Physiol       Date:  2014-06       Impact factor: 6.384

9.  Rapid visualisation of microarray copy number data for the detection of structural variations linked to a disease phenotype.

Authors:  Ian M Carr; Christine P Diggle; Kamron Khan; Chris Inglehearn; Martin McKibbin; David T Bonthron; Alexander F Markham; Rashida Anwar; Angus Dobbie; Sergio D J Pena; Manir Ali
Journal:  PLoS One       Date:  2012-08-17       Impact factor: 3.240
  9 in total

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