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Literature DB >> 5639915

Studies on extracellular ribonucleases of Ustilago sphaerogena. Purification and properties.

Abstract

1. Four ribonucleases were isolated from culture media of Ustilago sphaerogena. They were designated ribonucleases U(1), U(2), U(3) and U(4). 2. They were purified about 1600-, 3700-, 1100- and 16-fold respectively. 3. It was shown by gel filtration that ribonucleases U(1), U(2) and U(3) have molecular weights about 10000 like ribonuclease T(1), and that ribonuclease U(4) is much larger. 4. Ribonucleases U(1), U(2) and U(3) are thermostable, but ribonuclease U(4) is not. 5. The pH optimum of ribonucleases U(1) and U(4) is pH8.0-8.5, and that of ribonucleases U(2) and U(3) is pH4.5.

Entities: Species

Mesh：

Substances：
Ribonucleases

Year: 1968 PMID： 5639915 PMCID： PMC1198548 DOI： 10.1042/bj1060601

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

6 in total

1. STUDIES ON A RIBONUCLEASE FROMUSTILAGO SPHAEROGENNA. II. SPECIFICITY OF THE ENZYME.

Authors: D G GLITZ; C A DEKKER
Journal: Biochemistry Date: 1964-10 Impact factor: 3.162

2. STUDIES ON A RIBONUCLEASE FROM USTILAGO SPHAEROGENA. I. PURIFICATION AND PROPERTIES OF THE ENZYME.

Authors: D G GLITZ; C A DEKKER
Journal: Biochemistry Date: 1964-10 Impact factor: 3.162

3. Extracellular ribonucleases from Bacillus subtilis. I. Crystallization and specificity.

Authors: S NISHIMURA
Journal: Biochim Biophys Acta Date: 1960-12-04

4. A deoxyribonuclease from calf spleen. I. Purification and properties.

Authors: J F KOERNER; R L SINSHEIMER
Journal: J Biol Chem Date: 1957-10 Impact factor: 5.157

5. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

6. Studies on extracellular ribonucleases of Ustilago sphaerogena. Characterization of substrate specificity with special reference to purine-specific ribonucleases.

Authors: T Arima; T Uchida; F Egami
Journal: Biochem J Date: 1968-02 Impact factor: 3.857

6 in total

10 in total

1. Evidence that genomic and antigenomic RNA self-cleaving elements from hepatitis delta virus have similar secondary structures.

Authors: S P Rosenstein; M D Been
Journal: Nucleic Acids Res Date: 1991-10-11 Impact factor: 16.971

2. The use of ribonuclease U2 in RNA sequence determination. Some corrections in the catalog of oligomers produced by ribonuclease T1 digestion of Escherichia coli 16S ribosomal RNA.

Authors: T Uchida; L Bonen; H W Schaup; B J Lewis; L Zablen; C Woese
Journal: J Mol Evol Date: 1974-02-28 Impact factor: 2.395

Review 3. Microbial ribonucleases (RNases): production and application potential.

Authors: E Esin Hameş; Tuğçe Demir
Journal: World J Microbiol Biotechnol Date: 2015-10-03 Impact factor: 3.312

4. Characterization of the primary structural homology between the 16s ribosomal RNAs of Escherichia coli and Bacillus megaterium by oligomer cataloging.

Authors: K J Pechman; C R Woese
Journal: J Mol Evol Date: 1972 Impact factor: 2.395

5. Inquiries into the structure-function relationship of ribonuclease T1 using chemically synthesized coding sequences.

Authors: M Ikehara; E Ohtsuka; T Tokunaga; S Nishikawa; S Uesugi; T Tanaka; Y Aoyama; S Kikyodani; K Fujimoto; K Yanase
Journal: Proc Natl Acad Sci U S A Date: 1986-07 Impact factor: 11.205

6. Studies on extracellular ribonucleases of Ustilago sphaerogena. Characterization of substrate specificity with special reference to purine-specific ribonucleases.

Authors: T Arima; T Uchida; F Egami
Journal: Biochem J Date: 1968-02 Impact factor: 3.857

7. Control by cesium and intermediates of the citric acid cycle of extracellular ribonuclease and other enzymes involved in the assimilation of nitrogen.

Authors: W K Holloman; C A Dekker
Journal: Proc Natl Acad Sci U S A Date: 1971-09 Impact factor: 11.205

Studies on extracellular ribonucleases of Ustilago sphaerogena. Purification and properties.

1. STUDIES ON A RIBONUCLEASE FROMUSTILAGO SPHAEROGENNA. II. SPECIFICITY OF THE ENZYME.

2. STUDIES ON A RIBONUCLEASE FROM USTILAGO SPHAEROGENA. I. PURIFICATION AND PROPERTIES OF THE ENZYME.

3. Extracellular ribonucleases from Bacillus subtilis. I. Crystallization and specificity.

4. A deoxyribonuclease from calf spleen. I. Purification and properties.

5. Protein measurement with the Folin phenol reagent.

6. Studies on extracellular ribonucleases of Ustilago sphaerogena. Characterization of substrate specificity with special reference to purine-specific ribonucleases.

1. Evidence that genomic and antigenomic RNA self-cleaving elements from hepatitis delta virus have similar secondary structures.

2. The use of ribonuclease U2 in RNA sequence determination. Some corrections in the catalog of oligomers produced by ribonuclease T1 digestion of Escherichia coli 16S ribosomal RNA.

Review 3. Microbial ribonucleases (RNases): production and application potential.

4. Characterization of the primary structural homology between the 16s ribosomal RNAs of Escherichia coli and Bacillus megaterium by oligomer cataloging.

5. Inquiries into the structure-function relationship of ribonuclease T1 using chemically synthesized coding sequences.

6. Studies on extracellular ribonucleases of Ustilago sphaerogena. Characterization of substrate specificity with special reference to purine-specific ribonucleases.

7. Control by cesium and intermediates of the citric acid cycle of extracellular ribonuclease and other enzymes involved in the assimilation of nitrogen.

8. Comparison of the primary structures of ribonuclease U2 isoforms.

9. Polyadenylation of vesicular stomatitis virus mRNA.

10. Improving RNA modification mapping sequence coverage by LC-MS through a nonspecific RNase U2-E49A mutant.