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Literature DB >> 5635382

Studies on the pathogenesis of fever. XVI. Purification and further chemical characterization of granulocytic pyrogen.

M S Kozak, H H Hahn, W J Lennarz, W B Wood.

Abstract

Small quantities of highly purified granulocytic pyrogen have been separated from contaminating proteins by disc electrophoresis in polyacrylamide gel. The biologically active material thus isolated was shown to be electrophoretically homogeneous at pH 9 and pH 3.8. Earlier work on the chemical properties of the pyrogen molecule has been extended to include: (a) estimation of its molecular weight by gel filtration; (b) demonstration of free sulfhydryl groups essential for its biological activity; and (c) evidence that it is not inactivated by exhaustive extraction with ethanolether or n-heptane.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1968 PMID： 5635382 PMCID： PMC2138451 DOI： 10.1084/jem.127.2.341

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

28 in total

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors: B J DAVIS
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

2. DISC ELECTROPHORESIS IN POLYACRYLAMIDE GELS: EXTENSION TO NEW CONDITIONS OF PH AND BUFFER.

Authors: D E WILLIAMS; R A REISFELD
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

3. Studies on the pathogenesis of fever. VIII. Fever-producing substances in the serum of dogs.

Authors: R G PETERSDORF; I L BENNETT
Journal: J Exp Med Date: 1957-08-01 Impact factor: 14.307

4. Studies on the pathogenesis of fever. V. The relation of circulating endogenous pyrogen to the fever of acute bacterial infections.

Authors: M K KING; W B WOOD
Journal: J Exp Med Date: 1958-02-01 Impact factor: 14.307

5. Studies on the pathogenesis of fever with influenzal viruses. I. The appearance of an endogenous pyrogen in the blood following intravenous injection of virus.

Authors: E ATKINS; W C HUANG
Journal: J Exp Med Date: 1958-03-01 Impact factor: 14.307

6. Studies on the pathogenesis of fever. VII. Preliminary chemical characterization of leucocytic pyrogen.

Authors: G W RAFTER; R D COLLINS; W B WOOD
Journal: J Exp Med Date: 1960-06-01 Impact factor: 14.307

7. Studies on the pathogenesis of fever. XI. Quantitative features of the febrile response to leucocytic pyrogen.

Authors: D L BORNSTEIN; C BREDENBERG; W B WOOD
Journal: J Exp Med Date: 1963-03-01 Impact factor: 14.307

8. Studies on the pathogenesis of fever. X. The effect of certain enzyme inhibitors on the production and activity of leucocvtic pvrogen.

Authors: H K KAISER; W B WOOD
Journal: J Exp Med Date: 1962-01-01 Impact factor: 14.307

9. Studies on the pathogenesis of fever. XIV. Further observations on the chemistry of leukocytic pyrogen.

Authors: G W Rafter; S F Cheuk; D W Krause; W B Wood
Journal: J Exp Med Date: 1966-03-01 Impact factor: 14.307

10. STUDIES ON THE PATHOGENESIS OF FEVER. XII. ELECTROLYTIC FACTORYS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUKOCYTES.

Authors: R D BERLIN; W B WOOD
Journal: J Exp Med Date: 1964-05-01 Impact factor: 14.307

16 in total

1. Studies on the mechanism of endogenous pyrogen production. II. Role of cell products in the regulation of pyrogen release from blood leukocytes.

Authors: P Bodel
Journal: Infect Immun Date: 1974-09 Impact factor: 3.441

Studies on the pathogenesis of fever. XVI. Purification and further chemical characterization of granulocytic pyrogen.

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

2. DISC ELECTROPHORESIS IN POLYACRYLAMIDE GELS: EXTENSION TO NEW CONDITIONS OF PH AND BUFFER.

3. Studies on the pathogenesis of fever. VIII. Fever-producing substances in the serum of dogs.

4. Studies on the pathogenesis of fever. V. The relation of circulating endogenous pyrogen to the fever of acute bacterial infections.

5. Studies on the pathogenesis of fever with influenzal viruses. I. The appearance of an endogenous pyrogen in the blood following intravenous injection of virus.

6. Studies on the pathogenesis of fever. VII. Preliminary chemical characterization of leucocytic pyrogen.

7. Studies on the pathogenesis of fever. XI. Quantitative features of the febrile response to leucocytic pyrogen.

8. Studies on the pathogenesis of fever. X. The effect of certain enzyme inhibitors on the production and activity of leucocvtic pvrogen.

9. Studies on the pathogenesis of fever. XIV. Further observations on the chemistry of leukocytic pyrogen.

10. STUDIES ON THE PATHOGENESIS OF FEVER. XII. ELECTROLYTIC FACTORYS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUKOCYTES.

1. Studies on the mechanism of endogenous pyrogen production. II. Role of cell products in the regulation of pyrogen release from blood leukocytes.

2. Studies on the pathogenesis of fever. XIX. Localization of pyrogen in granulocytes.

Review 3. Molecular mechanisms in endotoxin fever.

4. Rabbit macrophages secrete two biochemically and immunologically distinct endogenous pyrogens.

5. Studies on the active site of human leukocytic pyrogen.

6. The febrile responses in rabbits and rats to leucocyte pyrogens of different species.

7. Further comparisons of endogenous pyrogens and leukocytic endogenous mediators.

8. Mechanism of fever induction in rabbits.

9. Partial purification of human leukocytic pyrogen.

10. Studies on the pathogenesis of fever. 18. Activation of leukocytes for pyrogen production.