Literature DB >> 5621472

Characterization of an infectivity assay for the ribonucleic acid of bacteriophage MS2.

J H Strauss, R L Sinsheimer.   

Abstract

An infectivity assay for MS2 ribonucleic acid (RNA), which uses bacterial spheroplasts of an F(-) strain of Escherichia coli, has an efficiency of 10(-5) infected spheroplasts per RNA molecule. The characteristics of this assay and the influence of several parameters are presented. Important variables include the duration of exposure of the cells to lysozyme-ethylenediaminetetraacetic acid, the duration of exposure of the spheroplast stock to the RNA solutions before dilution, the concentration of RNA, and the presence of competing RNA. The growth kinetics of the virus in the infected spheroplasts and the extent of lysis have also been studied.

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Year:  1967        PMID： 5621472      PMCID： PMC375345     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  11 in total

1.  THE REPLICATION OF BACTERIOPHAGE MS2. II. AN IN VIVO SPECIFICITY FOR THE SYNTHESIS OF INFECTIVE RNA.

Authors:  J E DAVIS; D PFEIFER; R L SINSHEIMER
Journal:  J Mol Biol       Date:  1964-10       Impact factor: 5.469

2.  THE RELEASE OF RIBONUCLEASE INTO THE MEDIUM WHEN ESCHERICHIA COLI CELLS ARE CONVERTED TO SPEROPLASTS.

Authors:  H C NEU; L A HEPPEL
Journal:  J Biol Chem       Date:  1964-11       Impact factor: 5.157

3.  A MODIFIED METHOD OF MS2-RNA ASSAY.

Authors:  J H STRAUSS
Journal:  J Mol Biol       Date:  1964-12       Impact factor: 5.469

4.  The replication of bacteriophage MS2. 1. Transfer of parental nucleic acid to progeny phage.

Authors:  J E DAVIS; R L SINSHEIMER
Journal:  J Mol Biol       Date:  1963-03       Impact factor: 5.469

5.  Factors affecting the plaqueyield resulting from infection of E-coli K12 protoplasts by phenol extracts of the RNA-phage ft5.

Authors:  P KNOLLE; F KAUDEWITZ
Journal:  Biochem Biophys Res Commun       Date:  1962-10-17       Impact factor: 3.575

6.  Assay of infectious RNA from bacteriophage R 17.

Authors:  W PARANCHYCH
Journal:  Biochem Biophys Res Commun       Date:  1963-04-02       Impact factor: 3.575

7.  Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174.

Authors:  G D GUTHRIE; R L SINSHEIMER
Journal:  Biochim Biophys Acta       Date:  1963-06-25

8.  Purification of viral RNA by means of bentonite.

Authors:  H FRAENKEL-CONRAT; B SINGER; A TSUGITA
Journal:  Virology       Date:  1961-05       Impact factor: 3.616

9.  The replication of bacteriophage MS2. VI. Interaction between bacteriophage RNA and cellular components in MS2-infected Escherichia coli.

Authors:  G N Godson; R L Sinsheimer
Journal:  J Mol Biol       Date:  1967-02-14       Impact factor: 5.469

10.  Isolation and characterization of ribonuclease I mutants of Escherichia coli.

Authors:  R F Gesteland
Journal:  J Mol Biol       Date:  1966-03       Impact factor: 5.469
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  5 in total

Review 1.  Transfection of Enterobacteriaceae and its applications.

Authors:  R Benzinger
Journal:  Microbiol Rev       Date:  1978-03

2.  Reversion of Q beta RNA phage mutants by homologous RNA recombination.

Authors:  K Palasingam; P N Shaklee
Journal:  J Virol       Date:  1992-04       Impact factor: 5.103

3.  The in vitro addition of a polyadenylate sequence to the 3' end of phage Qbeta RNA and the biological activity of the product.

Authors:  C Gilvarg; F J Bollum; C Weissmann
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

4.  Use of miracil D to suppress bacterial ribonucleic acid and protein synthesis during bacteriophage MS2 infection.

Authors:  J H Cramer; R L Sinsheimer
Journal:  J Virol       Date:  1972-02       Impact factor: 5.103

Review 5.  Translation of the genome of a ribonucleic acid bacteriophage.

Authors:  M Kozak; D Nathans
Journal:  Bacteriol Rev       Date:  1972-03
  5 in total

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