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Literature DB >> 1054825

The in vitro addition of a polyadenylate sequence to the 3' end of phage Qbeta RNA and the biological activity of the product.

Abstract

Terminal riboadenylate transferase, purified from calf thymus, has been used to add a poly(A) extension to the 3' end of Qbeta RNA. The modified Qbeta RNA retains full infectivity in a spheroplast assay system. However, the progeny viruses do not contain poly(A) termini, indicating an in vivo rectification of the in vitro alteration.

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Year: 1975 PMID： 1054825 PMCID： PMC432324 DOI： 10.1073/pnas.72.2.428

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

1. Biogenesis of mRNA: genetic regulation in mammalian cells.

Authors: J E Darnell; W R Jelinek; G R Molloy
Journal: Science Date: 1973-09-28 Impact factor: 47.728

Review 2. Structure and function of phage RNA.

Authors: C Weissmann; M A Billeter; H M Goodman; J Hindley; H Weber
Journal: Annu Rev Biochem Date: 1973 Impact factor: 23.643

2. Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.

Authors: I W Mattaj; R Zeller
Journal: EMBO J Date: 1983 Impact factor: 11.598

2 in total

The in vitro addition of a polyadenylate sequence to the 3' end of phage Qbeta RNA and the biological activity of the product.

1. Biogenesis of mRNA: genetic regulation in mammalian cells.

Review 2. Structure and function of phage RNA.

3. Polyadenylate synthesis by extracts from L cells infected with vesicular stomatitis virus.

4. Association of polyadenylic acid with messenger RNA of vesicular stomatitis virus.

5. Transcription of in vitro polyadenylated ribonucleic acid with reverse transcriptase.

6. Polyadenylation of maternal RNA of sea urchin eggs after fertilization.

7. Terminal riboadenylate transferase: a polyadenylate polymerase from calf thymus gland.

8. Heterogeneity in the poly(A) content of the genome of Sindbis virus.

9. Use of viruses as carriers of added genetic information.

10. Polyadenylic acid sequences in the virion RNA of poliovirus and Eastern Equine Encephalitis virus.

1. Reversion of Q beta RNA phage mutants by homologous RNA recombination.

2. Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.