| Literature DB >> 558652 |
H Hirumi, J J Doyle, K Hirumi.
Abstract
Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37 degrees C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces.Entities:
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Year: 1977 PMID： 558652 DOI： 10.1126/science.558652
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728