| Literature DB >> 303956 |
H Hirumi, J J Doyle, K Hirumi.
Abstract
Animal-infective forms of Trypanosoma brucei (Strain 427) were successfully propagated in HEPES-buffered RPMI 1640 medium in the presence of bovine fibroblast-like cells for over 310 days. The organisms grown in this system were morphologically identical to the long slender bloodstream forms, retained their infectivity for mammalian hosts, and displayed variant-antigen on their surface. Technical details for establishing such bloodstream form cultures are described in the present paper.Entities:
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Year: 1977 PMID： 303956 PMCID： PMC2366732
Source DB: PubMed Journal: Bull World Health Organ ISSN： 0042-9686 Impact factor: 9.408