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Literature DB >> 55401

The histochemical application of dansylhydrazine as a fluorescent labeling reagent for sialic acid in cellular glycoconjugates.

Abstract

A new method for the fluorescent staining of stalic acid-containing glycoconjugates in fixed tissues is described. The procedure uses mild periodate oxidation, followed by condensation with dansylhydrazine and reduction of the hydrazones to hydrazines. The specificity of the reaction for sialic acid is tested on model glycoconjugates. The procedure gives superior resolution in comparison to the standard periodate Schiff procedure for cellular carbohydrates.

Entities: Chemical

Mesh：

Substances：

Year: 1975 PMID： 55401 DOI： 10.1007/bf00492629

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

10 in total

1. Introduction of a fluorescent label into the carbohydrate moiety of glycoconjugates.

Authors: P Weber; L Hof
Journal: Biochem Biophys Res Commun Date: 1975-08-18 Impact factor: 3.575

2. Modification of sialyl residues of sialoglycoprotein(s) of the human erythrocyte surface.

Authors: T H Liao; P M Gallop; O O Blumenfeld
Journal: J Biol Chem Date: 1973-12-10 Impact factor: 5.157

3. Fluorescent-labelled aprotinin: a new reagent for the histochemical detection of acid mucosubstances.

Authors: J A Kiernan; R W Stoddart
Journal: Histochemie Date: 1973

4. Microspectrofluorometry of a periodic acid-schiff reaction in blood cells.

Authors: X Yataghanas; G Gahrton; B Thorell
Journal: Exp Cell Res Date: 1969-07 Impact factor: 3.905

5. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes.

Authors: C G Gahmberg; S I Hakomori
Journal: J Biol Chem Date: 1973-06-25 Impact factor: 5.157

6. Topographical distribution of complex carbohydrates in the erythrocyte membrane.

Authors: T L Steck; G Dawson
Journal: J Biol Chem Date: 1974-04-10 Impact factor: 5.157

7. N-acetylneuraminic acid analogues. I. Preparation of the 8-carbon and 7-carbon compounds.

Authors: R L McLean; M Suttajit; J Beidler; R J Winzler
Journal: J Biol Chem Date: 1971-02-10 Impact factor: 5.157

8. The pattern of binding of fluorescein-labeled concanavalin A to the motile lymphocyte.

Authors: C W Smith; J C Hollers
Journal: J Reticuloendothel Soc Date: 1970-11

9. Action of concanavalin A on the attachment stage of phagocytosis by macrophages.

Authors: J M Allen; G M Cook; A R Poole
Journal: Exp Cell Res Date: 1971-10 Impact factor: 3.905

10. The gas chromatographic properties of biologically important N-acetylglucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides.

Authors: T Bhatti; R E Chambers; J R Clamp
Journal: Biochim Biophys Acta Date: 1970-11-24

10 in total

1. [Demonstration of an alkali PAS-effect using periodic acid at low concentration (author's transl)].

Authors: C Klessen
Journal: Histochemistry Date: 1978-07-12

2. Periodate oxidation of glycolipids: a borohydride-periodate-Schiff method for ganglioside demonstration in tissue sections.

Authors: S J Buk; O B High
Journal: Histochem J Date: 1986-05

3. How selective are the 'selective periodate oxidation' methods used in sialic acid histochemistry?

Authors: J Needham; P E Reid; D A Owen
Journal: Histochem J Date: 1991-06

4. Histochemical staining of sulphated and non-sulphated sialomucin in intestinal epithelium: practical difficulties associated with the KOH-AB 1.0-PAPS method.

Authors: S J Buk; M I Filipe
Journal: Histochem J Date: 1986-10

5. Simple procedure for assessing relative quantities of neutral and acidic sugars in mucin glycoproteins: its use in assessing cyclical changes in cervical mucins.

Authors: C B Gilks; P E Reid; P B Clement; D A Owen
Journal: J Clin Pathol Date: 1988-09 Impact factor: 3.411

9. Sialic acid in colonic mucin: an evaluation of modified PAS reactions in single and combination histochemical procedures.

Authors: R Roe; A P Corfield; R C Williamson
Journal: Histochem J Date: 1989-04

Review 10. Fluorescently labelled glycans and their applications.

Authors: Hongbin Yan; Ravi Shekar Yalagala; Fengyang Yan
Journal: Glycoconj J Date: 2015-08-04 Impact factor: 2.916

10 in total

The histochemical application of dansylhydrazine as a fluorescent labeling reagent for sialic acid in cellular glycoconjugates.

1. Introduction of a fluorescent label into the carbohydrate moiety of glycoconjugates.

2. Modification of sialyl residues of sialoglycoprotein(s) of the human erythrocyte surface.

3. Fluorescent-labelled aprotinin: a new reagent for the histochemical detection of acid mucosubstances.

4. Microspectrofluorometry of a periodic acid-schiff reaction in blood cells.

5. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes.

6. Topographical distribution of complex carbohydrates in the erythrocyte membrane.

7. N-acetylneuraminic acid analogues. I. Preparation of the 8-carbon and 7-carbon compounds.

8. The pattern of binding of fluorescein-labeled concanavalin A to the motile lymphocyte.

9. Action of concanavalin A on the attachment stage of phagocytosis by macrophages.

10. The gas chromatographic properties of biologically important N-acetylglucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides.

1. [Demonstration of an alkali PAS-effect using periodic acid at low concentration (author's transl)].

2. Periodate oxidation of glycolipids: a borohydride-periodate-Schiff method for ganglioside demonstration in tissue sections.

3. How selective are the 'selective periodate oxidation' methods used in sialic acid histochemistry?

4. Histochemical staining of sulphated and non-sulphated sialomucin in intestinal epithelium: practical difficulties associated with the KOH-AB 1.0-PAPS method.

5. Simple procedure for assessing relative quantities of neutral and acidic sugars in mucin glycoproteins: its use in assessing cyclical changes in cervical mucins.

6. Skeletal-muscle sarcolemma from normal and dystrophic mice. Isolation, characterization and lipid composition.

7. Histochemical studies of the mechanism of the periodic acid-phenylhydrazine-Schiff (PAPS) procedure.

8. The fluorescent demonstration of tissue aldehydes with dansylhydrazine.

9. Sialic acid in colonic mucin: an evaluation of modified PAS reactions in single and combination histochemical procedures.

Review 10. Fluorescently labelled glycans and their applications.