Literature DB >> 211102

[Demonstration of an alkali PAS-effect using periodic acid at low concentration (author's transl)].

C Klessen.   

Abstract

It has been shown that an isolated KOH PAS-effect of epithelial mucosubstances in the colonic mucosae of the rat can be demonstrated also without the conventionally used preliminary oxidation/reduction step. The method is based on the use of strongly diluted periodic acid after previous treatment of tissue sections with ethanolic KOH. As the positive reacting material has been proven to be sensitive against treatment with neuraminidase the modified KOH PAS-reaction should be related to the presence of acylated sialic acid residues in mucosubstances of the colonic mucosae of the rat.

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Year:  1978        PMID: 211102     DOI: 10.1007/bf00495991

Source DB:  PubMed          Journal:  Histochemistry        ISSN: 0301-5564


  18 in total

1.  A HISTOCHEMICAL COMPARISON OF HUMAN EPITHELIAL MUCINS IN NORMAL AND IN HYPERSECRETORY STATES INCLUDING PANCREATIC CYSTIC FIBROSIS.

Authors:  R LEV; S S SPICER
Journal:  Am J Pathol       Date:  1965-01       Impact factor: 4.307

2.  The effect of formalin on the periodic acid Schiff staining of certain types of mucus.

Authors:  A J HALE
Journal:  J Histochem Cytochem       Date:  1955-11       Impact factor: 2.479

3.  [Histochemical periodate reaction of reticulin and collagen fibers].

Authors:  W GRAUMANN
Journal:  Acta Histochem       Date:  1954       Impact factor: 2.479

4.  The apparent failure of sodium borohydride reduction to block further PAS reactivity in rat epithelial mucins.

Authors:  C F Culling; P E Reid
Journal:  Histochem J       Date:  1977-11

5.  Effect of modification of N-acetylneuraminic acid on the biological activity of human and ovine follicle-stimulating hormone.

Authors:  M Suttajit; L E Reichert; R J Winzler
Journal:  J Biol Chem       Date:  1971-05-25       Impact factor: 5.157

6.  Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins.

Authors:  L Van Lenten; G Ashwell
Journal:  J Biol Chem       Date:  1971-03-25       Impact factor: 5.157

7.  The histochemical demonstration of O-acylated sialic acid in gastrointestinal mucins. Their association with the potassium hydroxide-periodic acid-schiff effect.

Authors:  C F Culling; P E Reid; M G Clay; W L Dunn
Journal:  J Histochem Cytochem       Date:  1974-08       Impact factor: 2.479

8.  N-acetylneuraminic acid analogues. I. Preparation of the 8-carbon and 7-carbon compounds.

Authors:  R L McLean; M Suttajit; J Beidler; R J Winzler
Journal:  J Biol Chem       Date:  1971-02-10       Impact factor: 5.157

9.  Carbohydrate-rich compounds in the colonic mucosa of man. I. Histochemical characteristics of normal and adenomatous colonic mucosa.

Authors:  V Mäkelä; L K Korhonen; G Lilius
Journal:  Cancer       Date:  1971-01       Impact factor: 6.860

10.  Histochemical use of borohydrides as aldehyde blocking reagents.

Authors:  R D Lillie; P Pizzolato
Journal:  Stain Technol       Date:  1972-01
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  8 in total

1.  A new histochemical method for the selective periodate oxidation of total tissue sialic acids.

Authors:  D Volz; P E Reid; C M Park; D A Owen; W L Dunn
Journal:  Histochem J       Date:  1987 Jun-Jul

2.  Periodate oxidation of glycolipids: a borohydride-periodate-Schiff method for ganglioside demonstration in tissue sections.

Authors:  S J Buk; O B High
Journal:  Histochem J       Date:  1986-05

3.  How selective are the 'selective periodate oxidation' methods used in sialic acid histochemistry?

Authors:  J Needham; P E Reid; D A Owen
Journal:  Histochem J       Date:  1991-06

4.  Can 'mild' periodate oxidation be used for the specific histochemical identification of sialic acid residues?

Authors:  D Volz; P E Reid; C M Park; D A Owen; W L Dunn; C W Ramey
Journal:  Histochem J       Date:  1986-10

5.  Histochemical staining of sulphated and non-sulphated sialomucin in intestinal epithelium: practical difficulties associated with the KOH-AB 1.0-PAPS method.

Authors:  S J Buk; M I Filipe
Journal:  Histochem J       Date:  1986-10

6.  Simple procedure for assessing relative quantities of neutral and acidic sugars in mucin glycoproteins: its use in assessing cyclical changes in cervical mucins.

Authors:  C B Gilks; P E Reid; P B Clement; D A Owen
Journal:  J Clin Pathol       Date:  1988-09       Impact factor: 3.411

7.  Histochemical identification of side chain substituted O-acylated sialic acids: the PAT-KOH-Bh-PAS and the PAPT-KOH-Bh-PAS procedures.

Authors:  P E Reid; W L Dunn; C W Ramey; E Coret; L Trueman; M G Clay
Journal:  Histochem J       Date:  1984-06

Review 8.  The relevance of the aldehyde bisulfite toluidine blue reaction and its variants in the submicroscopic carbohydrate research.

Authors:  Josef Makovitzky; Susann Richter
Journal:  Acta Histochem       Date:  2009-01-20       Impact factor: 2.479

  8 in total

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