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Literature DB >> 55377

Cytochemical study of pseudoisocyanine stained human chromosomes.

A M Vagner-Capodano, M H Pinna-Delgrossi, A Stahl.

Abstract

Human meiotic and mitotic chromosomes were studied with N-N' diethyl pseudoisocyanine stain. Following methylation and oxydation, the staining allowed microscopic observation of slides with both monochromatic light and fluorescence. In addition, stained preparations can be permanently conserved. Preceeded by diverse methods of chromosome denaturation or 5-BUDR incorporation, PIC lends itself to a large number of banding techniques. Cytochemical study of stained chromosomes demonstrated a certain PIC affinity for DNA although tests performed do not exclude the possibility of PIC reaction with certain proteins.

Entities: Chemical Species

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Year: 1976 PMID： 55377 DOI： 10.1007/bf00270394

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

12 in total

1. [MICROTECHNIC FOR THE STUDY OF HUMAN CHROMOSOMES FROM A HUMAN LEUKOCYTE CULTURE].

Authors: J DE GROUCHY; M ROUBIN; E PASSAGE
Journal: Ann Genet Date: 1964

2. [Announcement of a new histochemical method of DNA demonstration with a higher sensitivity than the Feulgen reaction].

Authors: G STERBA
Journal: Acta Biol Med Ger Date: 1963

Cytochemical study of pseudoisocyanine stained human chromosomes.

1. [MICROTECHNIC FOR THE STUDY OF HUMAN CHROMOSOMES FROM A HUMAN LEUKOCYTE CULTURE].

2. [Announcement of a new histochemical method of DNA demonstration with a higher sensitivity than the Feulgen reaction].

3. An air-drying method for first meiotic prophase preparations from mammalian ovaries.

4. [Research on the mechanism of histochemical methylation of acid mucins].

5. [A new technic of analysis of the human karyotype].

6. Localization of heterochromatin in human chromosomes.

7. [Studies on oocyte nuclei of amphibians and teleosts with pseudoisocyanine].

8. [A contribution to the study of the "methylation-saponification" mechanism in the histochemistry of acid mucins].

9. Differences in DNA composition along mammalian metaphase chromosomes.

10. [Fluorescence microscopic demonstration of DNA in lampbrush chromosomes with N,N'-diethylpseudoisocyanine chloride].

1. Chromosome banding: specification of structural features of dyes giving rise to G-banding.

2. R- and CT-banding of human chromosomes with basic fuchsin.