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Literature DB >> 5499968

The purification and chemical composition of the lipopolysaccharide of Pseudomonas alcaligenes.

Abstract

1. A method for obtaining lipopolysaccharide free from glycosaminopeptide from isolated cell walls of Pseudomonas alcaligenes is discussed. 2. About 70-75% of the lipopolysaccharide and 86-90% of the isolated lipid A have been accounted for in terms of identifiable components. 3. Hydrolysates of lipid A contain mainly inorganic phosphate, glucosamine, O-phosphorylglucosamine and fatty acids (dodecanoic acid, dodec-2-enoic acid, 3-hydroxydecanoic acid and 3-hydroxydodecanoic acid), of which the last is the main N-acylating acid of the glucosamine backbone. 4. Material corresponding to the polysaccharide moiety of the lipopolysaccharide is extensively degraded. 5. Solubilization of the lipopolysaccharide by using sodium deoxycholate appreciably affects the chemical composition of the material.

Entities: Chemical Species

Mesh：

Substances：
Amino Acids

Year: 1970 PMID： 5499968 PMCID： PMC1179637 DOI： 10.1042/bj1200559

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

21 in total

1. Biochemical studies on lipopolysaccharides of Salmonella R mutants. 6. Investigations on the structure of the lipid A component.

Authors: J Gmeiner; O Lüderitz; O Westphal
Journal: Eur J Biochem Date: 1969-01

2. Chemical studies on the lipid component of endotoxin, with special emphasis on its relation to biological activities.

Authors: N Kasai
Journal: Ann N Y Acad Sci Date: 1966-06-30 Impact factor: 5.691

3. The extraction of cell walls of Pseudomonas aeruginosa with aqueous phenol. Material from the phenol layer.

Authors: K Clarke; G W Gray; D A Reaveley
Journal: Biochem J Date: 1967-11 Impact factor: 3.857

4. The extraction of cell walls of Pseudomonas aeruginosa with aqueous phenol. The insoluble residue and material from the aqueous layers.

Authors: K Clarke; G W Gray; D A Reaveley
Journal: Biochem J Date: 1967-11 Impact factor: 3.857

5. The effect of ethylenediaminetetra-acetic acid on the cell walls of some gram-negative bacteria.

Authors: G W Gray; S G Wilkinson
Journal: J Gen Microbiol Date: 1965-06

6. Characterization of a protein-lipopolysaccharide complex released from cell walls of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid.

Authors: S W Rogers; H E Gilleland; R G Eagon
Journal: Can J Microbiol Date: 1969-07 Impact factor: 2.419

7. Quantitative determination of neutral monosaccharide residues in glycoproteins and glycopeptides by thin-layer densitometry.

Authors: W M Lamkin; D N Ward; E F Walborg
Journal: Anal Biochem Date: 1966-12 Impact factor: 3.365

8. Reaction of endotoxin and surfactants. I. Physical and biological properties of endotoxin treated with sodium deoxycholate.

Authors: E Ribi; R L Anacker; R Brown; W T Haskins; B Malmgren; K C Milner; J A Rudbach
Journal: J Bacteriol Date: 1966-11 Impact factor: 3.490

9. Polysaccharides in vegetative and aggregation-competent amoebae of Dictyostelium discoideum. 2. Purification and characterization of amoeba-degraded bacterial polysaccharides.

Authors: D Malchow; O Lüderitz; B Kickhöfen; O Westphal; G Gerisch
Journal: Eur J Biochem Date: 1969-01

10. The cell walls of Pseudomonas aeruginosa. General composition.

Authors: K Clarke; G W Gray; D A Reaveley
Journal: Biochem J Date: 1967-11 Impact factor: 3.857

19 in total

1. Nature, type of linkage, and absolute configuration of (hydroxy) fatty acids in lipopolysaccharides from Xanthomonas sinensis and related strains.

Authors: E T Rietschel; O Lüderitz; W A Volk
Journal: J Bacteriol Date: 1975-06 Impact factor: 3.490

2. O-antigen conversion in Pseudomonas aeruginosa PAO1 by bacteriophage D3.

Authors: J Kuzio; A M Kropinski
Journal: J Bacteriol Date: 1983-07 Impact factor: 3.490

3. The role of bivalent ions in the inactivation of bacteriophage phi X174 by lipopolysaccharide from Escherichia coli C.

Authors: E Rowatt
Journal: Biochem J Date: 1984-10-01 Impact factor: 3.857

The purification and chemical composition of the lipopolysaccharide of Pseudomonas alcaligenes.

1. Biochemical studies on lipopolysaccharides of Salmonella R mutants. 6. Investigations on the structure of the lipid A component.

2. Chemical studies on the lipid component of endotoxin, with special emphasis on its relation to biological activities.

3. The extraction of cell walls of Pseudomonas aeruginosa with aqueous phenol. Material from the phenol layer.

4. The extraction of cell walls of Pseudomonas aeruginosa with aqueous phenol. The insoluble residue and material from the aqueous layers.

5. The effect of ethylenediaminetetra-acetic acid on the cell walls of some gram-negative bacteria.

6. Characterization of a protein-lipopolysaccharide complex released from cell walls of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid.

7. Quantitative determination of neutral monosaccharide residues in glycoproteins and glycopeptides by thin-layer densitometry.

8. Reaction of endotoxin and surfactants. I. Physical and biological properties of endotoxin treated with sodium deoxycholate.

9. Polysaccharides in vegetative and aggregation-competent amoebae of Dictyostelium discoideum. 2. Purification and characterization of amoeba-degraded bacterial polysaccharides.

10. The cell walls of Pseudomonas aeruginosa. General composition.

1. Nature, type of linkage, and absolute configuration of (hydroxy) fatty acids in lipopolysaccharides from Xanthomonas sinensis and related strains.

2. O-antigen conversion in Pseudomonas aeruginosa PAO1 by bacteriophage D3.

3. The role of bivalent ions in the inactivation of bacteriophage phi X174 by lipopolysaccharide from Escherichia coli C.

4. Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO.

5. Cellular fatty acid composition of selected Pseudomonas species.

6. Cellular fatty acids of Alcaligenes and Pseudomonas species isolated from clinical specimens.

7. Studies of polysaccharide fractions from the lipopolysaccharide of Pseudomonas aeruginosa N.C.T.C. 1999.

8. Isolation and characterization of 2-keto-3-deoxyoctonate-lipid A from a heptose-deficient mutant of Escherichia coli.

9. Occurrence of branched-cahin hydroxy fatty acids in Pseudomonas maltophilia.

10. Further studies of the chemical composition of the lipopolysaccharide of Pseudomonas aeruginosa.