Literature DB >> 5493858

Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers.

R J Boegman, M J Crumpton.   

Abstract

Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene-maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose-myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60-100% of the antibody adsorbed by Sepharose-myoglobin, and 60-90% of the eluted protein was precipitable antibody.

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Year:  1970        PMID： 5493858      PMCID： PMC1179607          DOI： 10.1042/bj1200373

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  A WATER-INSOLUBLE POLYANIONIC DERIVATIVE OF TRYPSIN. I. PREPARATION AND PROPERTIES.

Authors:  Y LEVIN; M PECHT; L GOLDSTEIN; E KATCHALSKI
Journal:  Biochemistry       Date:  1964-12       Impact factor: 3.162

2.  The serum proteins in multiple myelomatosis.

Authors:  R A Kekwick
Journal:  Biochem J       Date:  1940-09       Impact factor: 3.857

3.  Components of the electron transport system in adrenal steroid hydroxylase. Isolation and properties of non-heme iron protein (adrenodoxin).

Authors:  T Kimura; K Suzuki
Journal:  J Biol Chem       Date:  1967-02-10       Impact factor: 5.157

4.  Isolation of antibodies to the C-terminal heptapeptide of myoglobin with a synthetic peptide.

Authors:  S J Givas; E R Centeno; M Manning; A H Sehon
Journal:  Immunochemistry       Date:  1968-05

5.  Exchange of heme among hemoglobins and between hemoglobin and albumin.

Authors:  H F Bunn; J H Jandl
Journal:  J Biol Chem       Date:  1968-02-10       Impact factor: 5.157

6.  Immunochemistry of sperm whale myoglobin. 3. Modification of the three tyrosine residues and their role in the conformation and differentiation of their roles in the antigenic reactivity.

Authors:  M Z Atassi
Journal:  Biochemistry       Date:  1968-09       Impact factor: 3.162

7.  The chemical attachment of chymotrypsin to water-insoluble polymers using 2-amino-4,6-dichloro-s-triazine.

Authors:  G Kay; M D Lilly
Journal:  Biochim Biophys Acta       Date:  1970-02-11

8.  The C-terminal antigenic site of sperm-whale myoglobin: the immunological activities of synthetic peptides related to the C-terminus of myoglobin.

Authors:  M J Crumpton; H D Law; R C Strong
Journal:  Biochem J       Date:  1970-03       Impact factor: 3.857

Review 9.  Water-insoluble derivatives of enzymes, antigens, and antibodies.

Authors:  I Silman; E Katchalski
Journal:  Annu Rev Biochem       Date:  1966       Impact factor: 23.643

10.  Carrier-bound proteins: properties of peroxidase bound to insoluble carboxymethylcellulose particles.

Authors:  N Weliky; F S Brown; E C Dale
Journal:  Arch Biochem Biophys       Date:  1969-04       Impact factor: 4.013
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  2 in total

1.  Observations on the use of solid-phase-coupled antibodies in the radioimmunoassay of human placental lactogen.

Authors:  J Gardner; G Bailey; T Chard
Journal:  Biochem J       Date:  1974-03       Impact factor: 3.857

2.  Biological activity of vegetalizing and neuralizing inducing factors after binding to BAC-cellulose and CNBr-sepharose.

Authors:  Hildegard Tiedemann; Jochen Born
Journal:  Wilehm Roux Arch Dev Biol       Date:  1978-12
  2 in total

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