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Literature DB >> 5475635

Plasminogen: purification from human plasma by affinity chromatography.

Abstract

Plasminogen was prepared from human plasma by affinity chromatography on L-lysine-substituted Sepharose. Thirty milligrams of plasminogen, with a specific activity of 100 caseinolytic units (Committee on Thrombolytic Agents) per milligram of nitrogen, were obtained from 340 milliliters of plasma. This corresponds to over 200-fold purification from plasma. Disc-gel electrophoresis at pH 8.3 indicated seven distinct bands, all of which contained activity.

Entities: Chemical Gene Species

Mesh：

Substances：
Plasminogen

Year: 1970 PMID： 5475635 DOI： 10.1126/science.170.3962.1095

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

348 in total

1. Crystal structure of native chicken fibrinogen at 5.5-A resolution.

Authors: Z Yang; I Mochalkin; L Veerapandian; M Riley; R F Doolittle
Journal: Proc Natl Acad Sci U S A Date: 2000-04-11 Impact factor: 11.205

2. Isolation and characterization of cell lines with reduced urokinase binding.

Authors: H K Lau; J M Teitel; M Kim
Journal: Clin Exp Metastasis Date: 2000 Impact factor: 5.150

3. Angiostatin binds ATP synthase on the surface of human endothelial cells.

Authors: T L Moser; M S Stack; I Asplin; J J Enghild; P Højrup; L Everitt; S Hubchak; H W Schnaper; S V Pizzo
Journal: Proc Natl Acad Sci U S A Date: 1999-03-16 Impact factor: 11.205

4. Fletcher factor deficiency. A diminished rate of Hageman factor activation caused by absence of prekallikrein with abnormalities of coagulation, fibrinolysis, chemotactic activity, and kinin generation.

Authors: A S Weiss; J I Gallin; A P Kaplan
Journal: J Clin Invest Date: 1974-02 Impact factor: 14.808

Plasminogen: purification from human plasma by affinity chromatography.

1. Crystal structure of native chicken fibrinogen at 5.5-A resolution.

2. Isolation and characterization of cell lines with reduced urokinase binding.

3. Angiostatin binds ATP synthase on the surface of human endothelial cells.

4. Fletcher factor deficiency. A diminished rate of Hageman factor activation caused by absence of prekallikrein with abnormalities of coagulation, fibrinolysis, chemotactic activity, and kinin generation.

5. Specific and nonspecific channels leading to proteolysis in antibody preparations.

6. Mechanism of dexamethasone inhibition of plasminogen activator in rat hepatoma cells.

7. Turnover of human and monkey plasma kininogens in rhesus monkeys.

8. Positive co-operative binding at two weak lysine-binding sites governs the Glu-plasminogen conformational change.

Review 9. The human plasma fibrinolytic system: regulation and control.

10. Expression of macrophage procoagulant activity in murine systemic lupus erythematosus.